NGS Discovery Pools

Days vs. weeks might mean everything

NGS Discovery Pools are high-fidelity, individually-synthesized, 5′-biotinylated oligos for targeted NGS. Proprietary DNA synthesis equipment permits rapid, high-quality synthesis of nucleic acids. These nucleic acid probes can be used for creating custom capture panels that can be optimized, expanded, and combined with other panels. Improvements to our established manufacturing process allow you to build custom panels quickly at a fraction of the cost of conventional custom panels.

Fast custom panel turnaround time
Affordable for any size panel
Easy design for even coverage across your targets

Ordering

NGS Discovery Pools

For information about pricing or to get a quote, please contact IDT sales.

Request consultation

For panel designs requiring expert analysis, please contact IDT support.

Custom design services

If you already have your custom panel design, upload your sequences.

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Product details

NGS Discovery Pools are pooled, high-fidelity, single-stranded DNA probes for targeted next generation sequencing. Create custom capture panels that can be optimized, expanded, and combined with other panels. Panels ship in as few as 5 business days (<50,000 probes).

Proprietary DNA synthesis equipment permits rapid, high-quality synthesis of nucleic acids. This platform is the same proprietary synthesis platform used to make IDT Ultramers. It uses an "extra rich" synthesis cycle for long oligos. Along with this refined synthesis cycle, these improvements to our already established manufacturing methods allow you to acquire longer, purer oligos for your research.

We can help you optimize your panel with NGS functional testing.

Shipping and storage recommendations:

Shipped in buffer at reaction-ready concentrations
Short-term storage, up to 6 weeks: room temperature (15–25°C) or 4°C
Long-term storage, at least 24 months: –20°C

Performance

Robust coverage for multiplexed captures

NGS Discovery Pools produce consistent coverage for a range of multiplexed captures (Figure 1).

Figure 1. NGS Discovery Pools show robust capture for single to 20-plex captures. The custom built Pan-Cancer Panel (424 kb) was used to capture different samples using the standard IDT protocol. 92.5% of the target space was covered between 0.5–2X the mean.

Even coverage across targets

NGS Discovery Pools were tested with libraries created from human genomic DNA (Coriell). 500 ng library per sample was used to capture using the standard IDT protocol. The enriched libraries were sequenced on a NextSeq^® instrument (Illumina) as indicated and standard Picard Metrics calculated. Data shown is the average for an 8-plex capture (Table 1).

Table 1. NGS Discovery Pools custom panel performance.

	Panel			Picard metrics
Name	Target size (kb)	Probe count	Genes targeted	Reads per sample (150 bp read length)	% selected bases	Mean target coverage	% target at 30+ X	Duplicate rate %
Cancer hotspot	52	525	10	100,000	84.3	98	99.8	2.0
Pan-cancer	468	4961	125	900,000	85.8	102	98.1	1.5
Pan-cancer plus	802	7820	127	1,300,000	85.5	98	96.8	1.4