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Guide RNAs

Streamline knockout screens with IDT’s synthetic gRNA libraries and make efficient CRISPR edits with gRNAs chemically modified to reduce immune response — synthesized to the highest standards for purity and quality.
comparison of a 2 part crispr guide rna and a single crispr guide rna

Two-part or single? Select the ideal gRNA for CRISPR-Cas9

The two-part gRNA (A) consists of a tracrRNA and crRNA duplex, while a sgRNA (B) includes a linker sequence that fuses the crRNA and tracrRNA into a single gRNA oligo.
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Helpful tips when choosing two-part vs. single gRNAs

 
Situation Guide RNA Suggestions
Limited budget, no other constraints Two-part guide RNA
High endo- or exo-nuclease activity in cells Single guide RNA (first choice)
OR (next choice if the first choice does not work), two-part guide RNA with many chemical modifications,*
OR (third choice, if the above choices do not work), single guide RNA with many chemical modifications**
Delivery of pre-formed Cas9 protein Two-part guide RNA, OR single guide RNA (both choices are equally likely to work)
Delivery of mRNA or plasmid coding for Cas9 Single guide RNA for longer stability
Low editing efficiency with single guide RNA Two-part guide RNA (first choice),
OR try another target site (second choice if the first choice does not work)
Low editing efficiency with two-part guide RNA Single guide RNA (first choice),
OR try another target site (second choice if the first choice does not work)
 
*The two-part guide RNA containing many chemical modifications is known as Alt-R CRISPR-Cas9 crRNA XT.
**Single guide RNA with many chemical modifications can be custom-ordered. However, even our standard sgRNAs contain some chemical modifications.

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CGMP gRNA products are manufactured in accordance with ICH Q7, 21CFR210, 211, and parts of 600. IDT engineering runs and CGMP gRNA are for development and investigational use only. The performance characteristics of this product have not been established.  This product is not intended to be used as final drug product. The purchaser is solely responsible for all decisions regarding the intended use of the product and any associated legal or regulatory obligations.