Custom Gene Synthesis Services

We store onboarded custom vectors for two years after the most recent order in that vector, at no additional fee.

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We are open to reviewing your vector if it does not meet the above criteria. Please contact your regional scientific applications support team with details of your vector, providing the sequence in FASTA format, if possible.

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During the initial phase of our launch, we’re prioritizing broad access to complex genes. To support as many customers as possible, these genes are currently available in IDT’s standard catalog vectors only. 

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We’re actively expanding the range of lengths and complexities available for online ordering. To ensure a high-quality customer experience, these capabilities are being rolled out in phases.

We will notify customers as new options become available. If you’re interested in ordering higher-complexity genes or constructs up to 50 kb today, please contact us for early access.

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Ansa specializes in manufacturing highly complex genes. All genes are delivered with a guaranteed minimum yield of 250 ng and typically ship at approximately 1.5 μg (non‑normalized).

Ansa DNA quantity is measured using a fluorescence‑based method (Qubit®, Thermo Fisher Scientific), which may report lower concentrations than absorbance‑based techniques (NanoDrop®, Thermo Fisher Scientific). If you have questions about yields or quantification, we’re happy to help — please contact us for more information.

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You can now order Ansa genes directly through our online ordering portal. Each gene is automatically routed to the optimal IDT or Ansa manufacturing workflow based on length and complexity, delivering the best balance of speed and cost.

Click here to place your order now.

For sequences that exceed the current online limits, please contact us to access higher‑complexity designs and gene lengths up to 50 kb.

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IDT Genes and Mini Gene™ sequences are delivered as double-stranded DNA, cloned into your provided custom vector.

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Please provide the 50 bases of sequence flanking the insertion site you wish us to use when submitting a new request through the Custom Vector intake tool. The vector's single fixed insertion site is defined by these flanking sequences. IDTs proprietary assembly method requires no restriction sites and can remove existing sequences from a vector. 

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Simple plasmid preps using a spin column or alkaline lysis method of purification are acceptable. Vectors greater than 8 kilobase pairs in size, those which require specialized cell lines or growth conditions for propagation, or those with exceptionally low yields may incur additional costs and/or turnaround time for gene production after validation.

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• An E. coli origin of replication
• Kanamycin, Ampicillin, or Chloramphenicol resistance marker
• 1 µg of circular plasmid

A minimum of 50 bases of sequence flanking the insertion site you would like us to use for the assembly of your gene inserts into the vector. Therefore, you do not need to specify restriction sites for assembly.

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Synthesis time depends on the length of the custom gene.

Length of custom gene	Time to ship after order confirmation (business days)
Up to 500 bp	8
501–5000 bp	12
More than 5000 bp	Inquire*

* Longer sequences may take additional time depending on their length and complexity. Contact us to submit sequences longer than 5000 bp for an official review and quote, including an estimated turnaround time.

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We can synthesize custom genes from 25 bp to more than 5 kb in length.

If you would like to submit a sequence for review or obtain an official quote, contact us.

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We have removed the multiple cloning site and most common restriction sites from our standard vectors.

Because of this, you should be able to add any convenient restriction sites to your sequences to facilitate subcloning. If you would like more information about our standard vectors, contact us.

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By not having a set multiple cloning site in our vectors, customers are able to choose the optimal restriction site set required to subclone into their choice of destination vector. You should add the restriction sites to your sequences when placing your order.

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IDT can synthesize genes in customer supplied vectors. Before we can accept orders in the custom vector, we will need to complete a validation process to confirm it is compatible with our synthesis process.

If you have questions about vector validation or would like an official quote, contact us for more details. The custom vector onboarding has a minimal fee associated, however, if the vector is accepted and successfully onboarded there are no additional fees for using a custom vector.

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IDT ships custom gene dry unless otherwise requested. While dry they should be stable up to 2 years. After resuspending in a high-quality, molecular-grade water or buffer, pH 7.5–8.0, you should store the DNA at –20°C, and if necessary, aliquot to avoid more than 2 or 3 freeze-thaw cycles.

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Plasmid DNA is fragmented prior to sequencing. Next, the fragmented DNA must be converted to a library that is compatible with the NGS sequencing platform, which is done using transposases to randomly add sequencing adapters to the fragments.

While this process is somewhat random, it is influenced by the structure and composition of the sequence.

Similarly, the success of individual reads within the run is also influenced by structure and sequence. Highly structured areas, areas with extremes in GC, and other complexities tend to affect read depths in areas where they occur.

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Even with clonally pure samples, NGS traces rarely show 100% sequence identity. This is because errors do occur during sequencing reactions and the subsequent data analysis.

These error rates are typically between 0.1% and 0.01%, but they can be locally elevated in regions of extreme GC content or secondary structure. Misalignment of the traces can also occur, particularly in highly repetitive sequences.

Finally, small subpopulations of sequence variants can arise during propagation of E. coli.

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To optimize manufacturing and delivery time, synthetic genes ≤5 kb in length will be provided in a “best-fit” IDT cloning vector (unless the Golden Gate vectors are selected). A choice of either ampicillin or kanamycin selection markers is available. Your high-quality, synthetic gene can then be subcloned into the vector of your choice using a variety of methods.

View a list of the IDT cloning vectors and sequence information. The identity of the cloning vector used, its sequence, and insertion site will be confirmed in the documentation accompanying the product.

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To optimize manufacturing and delivery time, synthetic genes ≤5 kb in length will be provided in a "best-fit" IDT cloning vector. A choice of either ampicillin or kanamycin selection markers is available. Your high-quality, synthetic gene can then be subcloned into the vector.

For more information, and a list of the IDT cloning vectors. The identity of the cloning vector used, its sequence, and insertion site will be confirmed in the documentation accompanying the product.

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