PrimeTime® qPCR Primer Assays

Primer-only premixed assays for analyzing gene expression using intercalating dyes

PrimeTime qPCR Primer Assays provide a primer pair designed for real-time PCR using intercalating dyes, such as SYBR® Green (Molecular Probes) or EvaGreen® (Biotium) dyes. Predesigned assays for human, mouse, or rat are designed with advanced bioinformatic and thermodynamic sequence analytics and for easy selection. Custom assays may be created for any sequence from any species using the PrimerQuest® tool.

  • Advanced bioinformatics delivers the best design possible, with considerations for exon location and number of transcripts detected
  • Achieve >90% efficiency over 4 or more orders of magnitude with our predesigned assays, guaranteed
  • Test target sequence amplification prior to using more expensive probe-based assays
  • Receive primer sequences with all orders