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DNA oligos

Consistently generate reliable data from high quality oligos.

All single-stranded and duplexed DNA sequences are produced with high coupling efficiencies, resulting in high quality DNA products. Our specialized platforms allow us to deliver primers for PCR, dual-labelled probes for qPCR, indexed adapters and fusion primers for sequencing, and a variety of custom products.

Ordering

  • Complete confidence in oligos that are checked by ESI-mass spectrometry*
  • Begin your project sooner with >90% of orders shipped within 24 hours**
  • Extensive control of oligo specifications with custom formulation and mixing options

* With the exception of mixed base oligos, which could potentially represent multiple sequences and therefore cannot be accurately evaluated by ESI mass spectrometry.
** small scale, standard, desalted oligos

Single-stranded DNA

Shipped dry, or resuspended to your specifications.

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For larger quantities, click here.

RxnReady® Primer Pools

2 oligos premixed in a single tube. Shipped dry, or resuspended to your specifications.

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The following mixing fee will be applied to each pool:

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Duplexed DNA

2 oligos, annealed and delivered in a single tube. Shipped dry.

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The following annealing fees will be applied to each duplex ordered:

100 nmol:

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250 nmol:

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1 µmol:

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Single-stranded DNA

Shipped dry, or resuspended to your specifications. A minimum of 24 and 96 oligos required for 96- and 384-well plates, respectively. 500 pmol scale requires a minimum of 96 oligos.

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Primer mix DNA

2 premixed oligos per well. Shipped dry or resuspended to your specifications. A minimum of 24 and 96 mixes is required for 96- and 384-well plates, respectively.

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Duplexed DNA

1 oligo pair per well, annealed. Shipped dry. A minimum of 24 and 96 oligo duplexes are required for 96- and 384-well plates, respectively.

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The following annealing fee will be applied to each plate of duplexed DNA:

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For ordering inquiries, please contact Contact us.

SameDay™ Oligos

Order today and receive your oligos in tubes on the following business day. Shipped dry, or resuspended to 100 µM in IDTE buffer, pH 8.0. Learn more »

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HotPlates® Oligo Service

Order today and receive your oligos in 2 business days. Delivered in 96-well, V-bottom plates. A minimum of 48 oligos is required per plate. Shipped dry. Learn more »

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The following service fee will be added to each plate:

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Rapid HPLC™ Oligos

Order today and receive your HPLC-purified oligos in 2 business days. Shipped dry in tubes. Learn more »

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Product Details

DNA oligos are short, single- and double-stranded synthetic DNA sequences that can be used in nearly any molecular biology application. Using the Oligo Entry ordering tool, you can design your oligos to contain any DNA sequence you require and select from hundreds of modifications to suit your specific research needs.

Every DNA oligo you receive will be deprotected and desalted to remove small molecule impurities. In addition, your oligos will be quality controlled via proprietary ESI-mass spectrometry methods *, and quantified twice by UV spectrophotometry to provide accurate yield measurements.

* With the exception of mixed base oligos, which could potentially represent multiple sequences and therefore cannot be accurately evaluated by ESI mass spectrometry.

For standard, desalted oligos ≥20 bases, we offer the following yield guarantees:

ProductAvailable lengthGuaranteed yield*
25 nmol DNA oligo15–60 bases10 nmol
100 nmol DNA oligo10–90 bases30 nmol
250 nmol DNA oligo5–100 bases50 nmol
1 µmol DNA oligo5–100 bases200 nmol
2 µmol DNA oligo5–100 bases400 nmol

5 µmol DNA oligo

5–100 bases1000 nmol
10 µmol DNA oligo5–100 bases2000 nmol

* Guaranteed yield for unmodified oligos 25–50 bases; will vary with oligo composition, length, and purification.

Product Data

IDT’s long-standing reputation as a leader and pioneer in custom oligo manufacturing is largely due to our vertically integrated proprietary synthesis platforms. Nearly every component of our manufacturing process is designed and developed in-house, including specialized synthesizers that can accommodate the most demanding oligo requests, and high-throughput automation systems that ensure the fastest turnaround times possible. We do not rely on third-party manufacturers for any of the machines or chemical reagents used for synthesis, allowing us to easily monitor and refine instruments and reagents with ease. Our ability to control these variables allows us to produce oligos that are unmatched in quality and consistency.

An important measure of quality for any oligo manufacturer is coupling efficiency, because this directly affects the amount of full-length product you receive. Our manufacturing processes achieve extremely high coupling efficiencies (Figure 1) and are standardized across our global manufacturing sites (Figure 2) to provide you with most consistent quality in the industry. No matter where in the world you are conducting your research, an IDT oligo is an IDT oligo. Our commitment to quality and consistency results in an extremely reliable product that will perform the same each time it is used. Because your research requires a steady supply of oligos over time, this reliability provides confidence in your research.

Comparison of IDT oligo coupling efficiency and other suppliers oligo coupling efficiency.

Figure 1. IDT proprietary platforms have a better coupling efficiency than other suppliers, which provides more full-length oligonucleotides in your order. Small increases in coupling efficiency (≤1%) result in measurable increases in full-length product yield. The curves represent the theoretical yield for different lengths of oligos based on a coupling efficiency of 99.4% (IDT oligos, n = 126) and 99.1% (other supplier, n = 134 from three different suppliers) using the formula, percent full length product = (eff )(n-1)*100 where eff = coupling efficiency (for example, 99.4% = 0.994) and (n–1) is the number of coupling reactions needed to make an oligo of length n.

Figure 2. IDT tracks coupling efficiency at our manufacturing sites to ensure consistent quality. Many variables can lead to reduced oligo quality, including ambient temperature, humidity, and input reagents. Monitoring of coupling efficiency at IDT manufacturing sites ensures that we provide our customers with high quality products. A quarterly average is shown 
(n = 16 per manufacturing site).

Services

The following services represent our most common offerings. If your project requires a service not listed here, contact Technical and Customer Support and our experts will work with you individually to accommodate your request.

Purification

For demanding applications such as multiplex PCR, cloning, mutagenesis, or antisense/RNAi methods, additional purification will significantly improve oligo performance. All purified oligos up to 60 bases receive a purity guarantee via QC by capillary electrophoresis. Learn more »

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Formulation

Different research needs and various individual preferences in the field require that oligos be delivered in unique formats. With the formulation services listed, you can have your oligos tailor-made to your specific requirements. Learn more »

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Custom quality control

In addition to the free ESI-mass spectrometry analysis provided for all oligos, your project may require one of the analytical services listed below. Learn more »

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Large-scale synthesis

Custom DNA oligos can be ordered in bulk with the same high purity, competitive pricing, and fast turnaround times you expect from IDT. To learn more about our large-scale synthesis capabilities, click here.

GMP and OEM

If you require oligos that are approved for use in molecular diagnostic applications, or if you are interested in our third-party manufacturing services, please click here.

Frequently asked questions

*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.

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