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Application

Best choice

Alternatives

Germline characterization with no concern for sample crosstalk
i.e., SNPs, indels, crude CNVs

UDI

SI, CDI, or UDI-UMI

Germline characterization where sample cross talk is important
i.e., translational research, inherited genetics, infectious disease

UDI

UDI-UMI

Low-level mutation detection, down to ~1% frequency
i.e., cancer, mosaicism, other diseases where somatic mutations are important

UDI-UMI

TruSeq™–Compatible Full-length Adapters

TruSeq™–Compatible Stubby Adapter

TruSeq™– or Nextera™–Compatible Indexing Primers

Delivered as

Duplex

Forward and reverse primer mix

Normalized to

15 μM

20 μM (10 μM each primer)

Volume delivered

16 reaction

80 μl

96 reaction

480 μl

2 nmol

130 μl

200 μl

8 nmol

530 μl

800 μl

24 nmol

1600 μl

2400 μl

Suggested amount/reaction to use (may vary by application)

75 pmol in 5 μl

50 pmol (per primer) in 5 μl

Introduction

IDT understands that the needs of genomics projects are not one-size-fits-all, and provides custom adapters that you can tailor to your needs. Our scientists have created this Custom Adapter Configurator tool to guide you through selection of several options as part of the adapter design process.

Consecutive, starting with index #1 and ending with index # Consecutive, starting with index # and ending with index #

NOTE: Index values should be between and

File: ( indexes)

Loading Indexes ...

Source

# Available

Index length

Blocker

Version

View indexes

IDT

Up to 384

8 bp

Standard

idt8-indexes.xlsx

IDT

Up to 3840

10 bp

Custom

idt10-indexes.xlsx

ILMN

Up to 96

8 bp

Standard

ilmn8-indexes.xlsx

ILMN

Up to 384

10 bp

Custom

Add

Summary

Your price for this order: $

Adapter details

Plate name prefix:

What you will receive

Building order...

Note: We suggest to avoid using custom adapters (such as those through the Custom Adapter Configurator Tool) and off-the-shelf, stocked adapters together in the same project. Please contact us for further assistance.

Need your adapters sooner? Check out our selection of pre-configured xGen™ UDI-UMI Adapters and xGen™ Stubby Adapter and UDI Primer Pairs in single-use plates.

RUO22-1362_001

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