You will be logged off in seconds due to inactivity. Click here to continue using this web page.

My Account Order History Wishlist LabLinker OligoCards Sign out

Products & Services

Oligos & Reagents

Custom DNA Oligos

Ultramer DNA Oligos

HotPlates Oligo Service

Rapid HPLC Oligos

TruGrade DNA Oligos

Affinity Plus DNA Oligonucleotides

Normalized Oligos

Custom DNA Oligos

Unlock new research paths with over 1400 modifications available.

Build Yours Now

Custom RNA Oligos

Ultramer RNA Oligos

Affinity Plus RNA Oligonucleotides

Custom DNA Oligos

Unlock new research paths with over 1400 modifications available.

Build Yours Now

Oligo Modifications

Attachment chemistry and linkers

Fluorophores and dark quenchers

ATTO Dyes Other Fluorophores Iowa Black® dark quenchers Additional dark quenchers

Phosphorylation

Click Chemistry Modifications

Phosphorothioate bonds

Custom DNA Oligos

Unlock new research paths with over 1400 modifications available.

Build Yours Now

Custom GMP Oligos

Nuclease Free Reagents

Nuclease Detection & Control

Custom DNA Oligos

Unlock new research paths with over 1400 modifications available.

Build Yours Now

Custom DNA Oligos

Unlock new research paths with over 1400 modifications available.

Build Yours Now

PCR / qPCR / dPCR

Custom qPCR & dPCR Probes

PrimeTime™ qPCR Probes

Affinity Plus™ qPCR Probes

PrimeTime™ LNA® qPCR Probes

Molecular Beacons

GMP Primers & Probes

MGB Eclipse® Probes

PrimeTime™ Pathogen Research Panels

Save time and money with PrimeTime Pathogen panels—flexible target selection, reliable results, and easy ordering.

Predesigned PCR-based Assays

PrimeTime™ Gene Expression Assays

PrimeTime™ Pathogen Research Panels

PrimeTime™ Sexual Health Research Panels

rhAmp™ SNP Genotyping System

PACE™ SNP Genotyping Assays

Predesigned Gene Expression Assay Tool

PrimeTime™ Pathogen Research Panels

Save time and money with PrimeTime Pathogen panels—flexible target selection, reliable results, and easy ordering.

Master Mixes & Reagents

PrimeTime™ Gene Expression Master Mix

PrimeTime™ One-Step 4X Broad-Range Master Mix

PrimeTime™ One-Step RT-qPCR Master Mix

rhAmp™ Genotyping Master Mix & Reporter Mixes

RNase H2 Enzyme

Oligo length standards

PrimeTime™ Pathogen Research Panels

Save time and money with PrimeTime Pathogen panels—flexible target selection, reliable results, and easy ordering.

Custom PCR Primers

RxnReady™ Primer Pools

PrimeTime™ qPCR Primer Assays

PrimeTime™ Pathogen Research Panels

Save time and money with PrimeTime Pathogen panels—flexible target selection, reliable results, and easy ordering.

ReadyMade Oligos

ReadyMade Primers

ReadyMade Random Hexamers

PrimeTime™ Pathogen Research Panels

Save time and money with PrimeTime Pathogen panels—flexible target selection, reliable results, and easy ordering.

PrimeTime™ Pathogen Research Panels

Save time and money with PrimeTime Pathogen panels—flexible target selection, reliable results, and easy ordering.

PrimeTime™ Sexual Health Research Panels

Optimized sexual health panels remove sequence design guesswork—saving time and money with flexible fluorophore options, platform compatibility, and reliable results.

CRISPR Gene Editing

CRISPR-Cas9 Systems

CRISPR-Cas12a (Cpf1) Systems

Cas9 Guide RNAs

Cas12a Guide RNAs

Custom CRISPR Guide RNA Libraries

Custom Guide RNAs

CGMP Guide RNA Manufacturing

CRISPR-Cas9 Systems

Optimized genome editing solutions for producing on-target, double-stranded DNA breaks.

CRISPR-Cas9 Systems

Optimized genome editing solutions for producing on-target, double-stranded DNA breaks.

HDR Donor Oligos

HDR Donor Blocks

CRISPR-Cas9 Systems

Optimized genome editing solutions for producing on-target, double-stranded DNA breaks.

rhAmpSeq™ CRISPR Data Analysis System

rhAmpSeq™ CRISPR Library Kit

rhAmpSeq™ CRISPR Panels

rhAmpSeq™ Index Primers

CRISPR-Cas9 Systems

Optimized genome editing solutions for producing on-target, double-stranded DNA breaks.

Genome Editing Detection Kit

CRISPR Off-target Analysis Services

CRISPR-Cas9 Systems

Optimized genome editing solutions for producing on-target, double-stranded DNA breaks.

CGMP gRNA Manufacturing

Accelerate your CRISPR therapeutics with our full CGMP guide RNA manufacturing/regulatory services.

Gene Synthesis & Fragments

gBlocks & gBlocks HiFi Gene Fragments

eBlocks Gene Fragments

Gene Synthesis Service

Megamer ssDNA DNA Fragments

Accelerate your high-throughput screening workflows with ready-to-use, NGS-verified clonal genes.

gBlocks HiFi Gene Fragments

Reduce screening time and minimize cloning errors with the only NGS-verified clonal gene fragment.

Next Generation Sequencing

Library Preparation

DNA Library Prep Kits

xGen DNA Library Prep Kits xGen cfDNA & FFPE DNA Library Prep Kit xGen ssDNA & Low-Input DNA Library Prep Kit

RNA Library Prep Kits

xGen Broad-Range RNA Library Prep Kit xGen RNA Library Prep Kit

Methyl-Seq Library Prep Kit

Your Next MasterMix

Bypass extraction, gain 5-plex sequencing, and maintain sensitivity, even with crude samples.

Hybridization Capture

xGen Predesigned Hyb Panels

xGen Exome Hyb Panel xGen Pan-Cancer Hyb Panel xGen AML Cancer Hyb Panel xGen Minimal Residual Disease (MRD) Hyb Panel xGen Inherited Disease Hyb Panel xGen CNV Backbone Hyb Panel xGen Human ID Hyb Panel xGen Human mtDNA Hyb Panel xGen SARS-CoV-2 Hyb Panel

xGen Custom Hyb Panels

xGen Hybridization Capture Core Reagents xGen Exome Sequencing Kit Trinity for Element

Your Next MasterMix

Bypass extraction, gain 5-plex sequencing, and maintain sensitivity, even with crude samples.

Amplicon Sequencing

xGen Oncology Amplicon Panels

xGen 56G Oncology Amplicon Panel xGen 57G Pan-Cancer Amplicon Panel xGen TP53 Amplicon Panel xGen EGFR Pathway Amplicon Panel xGen BRCA1/BRCA2 Amplicon Panel xGen BRCA1/BRCA2 PALB2 Amplicon Panel xGen Colorectal Amplicon Panel xGen Lung Amplicon Panel xGen Lynch Syndrome Amplicon Panel xGen Myeloid Amplicon Panel

xGen Inherited Disease Amplicon Panels

xGen CFTR Amplicon Panel

xGen Infectious Disease Amplicon Panels

xGen HIV Amplicon Panel xGen SARS-CoV-2 Amplicon Panel xGen Monkeypox Virus Amplicon Panel xGen Metagenomics Amplicon Panels xGen Respiratory Virus Amplicon Panel SARS-CoV-2 ARTIC Amplicon Panel SARS-CoV-2 Midnight Amplicon Panel

xGen Human Sample ID Amplicon Panel

xGen Custom Amplicon Panels

Your Next MasterMix

Bypass extraction, gain 5-plex sequencing, and maintain sensitivity, even with crude samples.

Library Normalization

xGen Normalase Module

xGen Pre-hybridization Capture Normalase Module

Your Next MasterMix

Bypass extraction, gain 5-plex sequencing, and maintain sensitivity, even with crude samples.

Anchored Multiplex PCR

T & B Cell RNA Assays

Your Next MasterMix

Bypass extraction, gain 5-plex sequencing, and maintain sensitivity, even with crude samples.

Archer Analysis

Adapters & Primers for NGS Sequencers

for Illumina Sequencers

for Ultima Genomics Sequencers

for Element Biosciences Sequencers

for DNBSEQ Sequencers

Your Next MasterMix

Bypass extraction, gain 5-plex sequencing, and maintain sensitivity, even with crude samples.

Sequencing Services

Your Next MasterMix

Bypass extraction, gain 5-plex sequencing, and maintain sensitivity, even with crude samples.

Custom Hyb Panels Design

We'll help you design fast, flexible target enrichment panels that fit into your NGS workflow.

Gene Regulation

RNA interference

Antisense Oligonucleotides

miRNA inhibitors

Antisense Oligonucleotides

Increase stability and reduce toxicity with nuclease-resistant mods and flexible chimeric designs.

DsiRNAs for RNAi

Effectively target cytoplasmic mRNA and noncoding RNA with predesigned or custom RNAi solutions.

GMP Oligo Manufacturing

GMP Primers & Probes

MGB Eclipse® Probes

Custom DNA Oligos

Unlock new research paths with over 1400 modifications available.

Build Yours Now

CGMP CRISPR Manufacturing

CGMP Guide RNAs

CGMP Cas Nucleases

CRISPR Off-target Analysis Services

CGMP gRNA Manufacturing

Accelerate your CRISPR therapeutics with our full CGMP guide RNA manufacturing/regulatory services.

CRISPR Off-target Analysis Services

Ensure the safety and efficacy of your CRISPR-based therapeutic with end-to-end services.

OEM & Additional Services

Assay Development

SciTools Plus API

Need Integration Help?

Let our team help with your e-commerce integration, punchout, portal, or API needs.

PAGE & HPLC Purification

Formulation & Packaging

Custom Quality Control

Need Integration Help?

Let our team help with your e-commerce integration, punchout, portal, or API needs.

Product Savings

All Products & Services

Custom DNA Oligos

>90% of standard desalted orders shipped within 24 hours. Quality checked by ESI-MS.

Accelerate your high-throughput screening workflows with ready-to-use, NGS-verified clonal genes.

Explore Solutions

Cancer Research

Cancer whole genome sequencing

Cancer exome sequencing

NGS cancer screening

Biomarker discovery

TMB and MSI biomarkers

Cancer liquid biopsy

Minimal residual disease research

Cancer Research Solutions

High performance NGS solutions with flexible, efficient workflows for your research insights.

Infectious Disease

Viral surveillance

Cancer Research Solutions

High performance NGS solutions with flexible, efficient workflows for your research insights.

Rare & Inherited Genetic Disease

Antibody Discovery

Protein Engineering

Cancer Research Solutions

High performance NGS solutions with flexible, efficient workflows for your research insights.

CRISPR Therapeutics eBook

Fundamental concepts, innovations, and insights about CRISPR R&D and the therapeutic landscape.

Research Techniques

Gene Expression

CRISPR Genome Editing

gBlocks HiFi Gene Fragments

Reduce screening time and minimize cloning errors with the only NGS-verified clonal gene fragment.

Synthetic Biology

Site Directed Mutagenesis

In Vitro Transcription

gBlocks HiFi Gene Fragments

Reduce screening time and minimize cloning errors with the only NGS-verified clonal gene fragment.

Next Generation Sequencing

Whole genome sequencing

Whole exome sequencing

Hybridization Capture

Amplicon Sequencing

Anchored Multiplex PCR Sequencing and Analysis

Targeted methyl sequencing

Whole transcriptome sequencing

gBlocks HiFi Gene Fragments

Reduce screening time and minimize cloning errors with the only NGS-verified clonal gene fragment.

gBlocks HiFi Gene Fragments

Reduce screening time and minimize cloning errors with the only NGS-verified clonal gene fragment.

Custom DNA Oligos

Unlock new research paths with over 1400 modifications available.

Build Yours Now

Get access to expert webinars, latest product updates, and promotions.

Support & Education

Frequently asked questions

User guides and protocols

Safety data sheets

Certificates of analysis

Archer support documents

How to: Quotes and Orders

Simplify your ordering process and quote requests with quick, easy, self-serve options.

Supplier information

How to: Quotes and Orders

Simplify your ordering process and quote requests with quick, easy, self-serve options.

DECODED online newsletter

More posts in DECODED

Coronavirus DECODED articles

CRISPR genome editing DECODED articles

Custom DNA & RNA Articles

Functional genomics articles

Genes and Gene fragments

Next generation sequencing articles

qPCR and PCR articles

Get access to expert webinars, latest product updates, and promotions.

Webinars & video tutorials

IDT Community Blog

Get access to expert webinars, latest product updates, and promotions.

Preferred service providers

Collab Network members

Get access to expert webinars, latest product updates, and promotions.

Oligos, PCR & qPCR

PCR Allele Competitive Extension (PACE) genotyping

SYBR Green dye assay and PrimeTime probe assays

Locked nucleic acids

Custom DNA Oligos

Unlock new research paths with over 1400 modifications available.

Build Yours Now

Real-Time PCR handbook

Learn how to design qPCR assays like a pro, get expert data analysis tips, and more.

Get the Handbook

CRISPR workflow

CRISPR proteins

CRISPR-Cas9 Systems

Optimized genome editing solutions for producing on-target, double-stranded DNA breaks.

CRISPR genome editing

Alt-R CRISPR-Cas systems

CRISPR-Cas9 genome editing

CRISPR-Cas12a (Cpf1) genome editing

CRISPR-Cas9 Systems

Optimized genome editing solutions for producing on-target, double-stranded DNA breaks.

CRISPR delivery

CRISPR detection

CRISPR off-target detection with NGS

CRISPR-Cas9 Systems

Optimized genome editing solutions for producing on-target, double-stranded DNA breaks.

CRISPR-Cas9 Systems

Optimized genome editing solutions for producing on-target, double-stranded DNA breaks.

Open up CRISPR editing to additional areas in genomes.

Synthetic Biology

Antibody applications

Accelerate your high-throughput screening workflows with ready-to-use, NGS-verified clonal genes.

DNA Cloning Guide

Learn about common DNA cloning techniques, sequence design considerations, and troubleshooting tips.

Get access to expert webinars, latest product updates, and promotions.

Oligo Design & Handling

OligoAnalyzer™ Tool

Analyzer definitions

Custom DNA Oligos

>90% of standard desalted orders shipped within 24 hours. Quality checked by ESI-MS.

Resuspension Calculator

Dilution Calculator

PrimerQuest Tool

Custom DNA Oligos

>90% of standard desalted orders shipped within 24 hours. Quality checked by ESI-MS.

CRISPR Genome Editing

Alt-R Predesigned Cas9 Selection Tool

Alt-R Custom Cas9 Design Tool

CRISPR-Cas9 Design Checker

Alt-R CRISPR HDR Design Tool

rhAmpSeq Design Tool

rhAmpSeq™ CRISPR Data Analysis Tool

CRISPR-Cas9 Systems

Optimized genome editing solutions for producing on-target, double-stranded DNA breaks.

Open up CRISPR editing to additional areas in genomes.

qPCR Assay Design

PrimerQuest™ Tool

Realtime PCR Tool

PrimeTime™ Predesigned Gene Expression Assays

PrimeTime Multiplex Dye Selection

Custom qPCR Probes

Multiplex your way with a wide range of high-efficiency quenchers, dyes, and scales.

Your Next MasterMix

Bypass extraction, gain 5-plex sequencing, and maintain sensitivity, even with crude samples.

Gene Regulation and RNAi

Predesigned DsiRNA Selection Tool

RNAi Design Tool

Antisense Oligonucleotides

Increase stability and reduce toxicity with nuclease-resistant mods and flexible chimeric designs.

DsiRNAs for RNAi

Effectively target cytoplasmic mRNA and noncoding RNA with predesigned or custom RNAi solutions.

rhAmp Genotyping Design Tool

Custom qPCR Probes

Multiplex your way with a wide range of high-efficiency quenchers, dyes, and scales.

Genes & Gene Fragments

Codon Optimization Tool

gBlocks Gene Fragments Entry Tool

Genes & Gene Fragments order status

Accelerate your high-throughput screening workflows with ready-to-use, NGS-verified clonal genes.

Library Concentration Conversion Calculator

Target Capture Probe Design & Ordering Tool

xGen™ Hyb Panel Design Tool

Custom Adapter Configurator Tool

NGS Solutions Builder Tool

Assay Marketplace

Your Next MasterMix

Bypass extraction, gain 5-plex sequencing, and maintain sensitivity, even with crude samples.

NGS Experts at Your Service

Reach your research goals faster with a consultation from our leading NGS specialists.

SciTools Plus API

Get access to expert webinars, latest product updates, and promotions.

Need Integration Help?

Let our team help with your e-commerce integration, punchout, portal, or API needs.

Company

General Information

Usage, warranty & licenses

About Us

Lorem ipsum dolor sit amet, consectetur adipiscing elitUt sed ornare leo

Get access to expert webinars, latest product updates, and promotions.

Choose your region, country/territory, and preferred language

Order Menu

Predesigned qPCR Assays

Loading Page...

Analyze gene expression with PrimeTime™ Predesigned qPCR Assays. Choose predesigned sequences for probe-based or intercalating dye-based assays for analysis of human, mouse, and rat transcriptomes.

Begin your project sooner—fast turn around times
Easily analyze and troubleshoot your experiments—disclosed primer and probe sequences

Step 1: Order Predesigned qPCR sequences
Step 2: Order Probe-Based qPCR Master Mix

Reference Gene Assays

Assay ID:
".g" suffix indicates that the assay may detect genomic DNA when using either PrimeTime qPCR Assays (5’ nuclease probe-based assays) or PrimeTime qPCR Primers.
".gs" suffix indicates that the assay ordered as PrimeTime qPCR Assays (5’ nuclease probe-based assays) will not detect gDNA. Assay ordered as PrimeTime qPCR Primers may detect genomic DNA.

Exon Location:
Exon boundaries are consolidated at the gene level whenever possible. Some exon locations may be marked with a superscripted 1, indicating assays in which consolidating at the gene level is not possible. For these assays, the exon location shown is related to the specific RefSeq listed in the results table.

Basic
Batch
Help

Gene Symbol

RefSeq

Assay ID

Species Human Mouse Rat

Default Assay Configuration 5' nuclease, probe included Intercalating dyes, primers only

Exact Match

Search Field

Species Human Mouse Rat

Default Assay Configuration 5' nuclease, probe included Intercalating dyes, primers only

Gene Symbol Search Batch Limit
On/Off

Exact Match

Assay ID:
".g" suffix indicates that the assay may detect genomic DNA when using either PrimeTime qPCR Assays (5’ nuclease probe-based assays) or PrimeTime qPCR Primers.
".gs" suffix indicates that the assay ordered as PrimeTime qPCR Assays (5’ nuclease probe-based assays) will not detect gDNA. Assay ordered as PrimeTime qPCR Primers may detect genomic DNA.

Exon Location:
Exon boundaries are consolidated at the gene level whenever possible. Some exon locations may be marked with a superscripted 1, indicating assays in which consolidating at the gene level is not possible. For these assays, the exon location shown is related to the specific RefSeq listed in the results table.

Compare Assays

.g = Assay may detect genomic DNA when using either PrimeTime qPCR Assays (5’ nuclease probe-based assays) or PrimeTime qPCR Primers.

.gs = Assay ordered as PrimeTime qPCR Assays (5’ nuclease probe-based assays) will not detect gDNA. Assay ordered as PrimeTime qPCR Primers may detect genomic DNA.

Scale

qPCR Assays (5' nuclease, probe included) Mini - 100 Reactions (20µL) Std - 500 Reactions (20µL) XL - 2500 Reactions (20µL) qPCR Primers (Intercalating dyes, primers only) Std – 500 Reactions (20uL)

Dye-Quencher Mod

N/A

Primer to Probe Ratio

2

2

2.5 nanomoles

Primer 1 Deliver Amount: 5.0 nanomoles

Primer 2 Deliver Amount: 5.0 nanomoles

N/A

Apply change to all rows

Processing...

Discover what's possible

800 328 2661

Applications

Cancer Research Infectious Disease Research Rare & Inherited Genetic Disease Research Antibody Discovery
Protein Engineering

Support & Education

User guides & protocols Safety data sheets Frequently asked questions Supplier information How to order and request a quote Vulnerability disclosure process Contact us

Company

About Leadership Sustainability Careers News Events

Products & Services

Oligos & Reagents PCR / qPCR / dPCR CRISPR Gene Editing Gene Synthesis & Fragments Next Generation Sequencing Gene Regulation GMP Oligo Manufacturing CGMP CRISPR Manufacturing OEM & Additional Services Product Savings

Tools

OligoAnalyzer Tool DNA Oligo Entry PrimerQuest Tool Predesigned qPCR Assays Predesigned CRISPR-Cas9 gRNA CRISPR HDR Design Tool gBlocks Gene Fragments Entry Codon Optimization Tool All tools

Follow us

Instagram
Facebook
X
Linkedin
Youtube

Preferences

Language: EN

Online Terms and Conditions of Sale
Privacy Policy Cookie Policy Payment Information
Do Not Sell or Share My Data
Biosecurity Attestation

© 2025 Integrated DNA Technologies, Inc.

Trademarks contained herein are the property of Integrated DNA Technologies, Inc. or their respective owners, and may be registered in the USA and/or other jurisdictions. For specific trademark information, see www.idtdna.com/trademarks. For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.