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Predesigned qPCR Assays
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Analyze gene expression with PrimeTime™ Predesigned qPCR Assays. Choose predesigned sequences for probe-based or intercalating dye-based assays for analysis of human, mouse, and rat transcriptomes.
- Begin your project sooner—fast turn around times
- Easily analyze and troubleshoot your experiments—disclosed primer and probe sequences
Step 1: Order Predesigned qPCR sequences
Step 2: Order Probe-Based qPCR Master Mix
Assay ID:
".g" suffix indicates that the assay may detect genomic DNA when using either PrimeTime qPCR Assays (5’ nuclease probe-based assays) or PrimeTime qPCR Primers.
".gs" suffix indicates that the assay ordered as PrimeTime qPCR Assays (5’ nuclease probe-based assays) will not detect gDNA. Assay ordered as PrimeTime qPCR Primers may detect genomic DNA.
Exon Location:
Exon boundaries are consolidated at the gene level whenever possible. Some exon locations may be marked with a superscripted 1, indicating assays in which consolidating at the gene level is not possible. For these assays, the exon location shown is related to the specific RefSeq listed in the results table.
".g" suffix indicates that the assay may detect genomic DNA when using either PrimeTime qPCR Assays (5’ nuclease probe-based assays) or PrimeTime qPCR Primers.
".gs" suffix indicates that the assay ordered as PrimeTime qPCR Assays (5’ nuclease probe-based assays) will not detect gDNA. Assay ordered as PrimeTime qPCR Primers may detect genomic DNA.
Exon Location:
Exon boundaries are consolidated at the gene level whenever possible. Some exon locations may be marked with a superscripted 1, indicating assays in which consolidating at the gene level is not possible. For these assays, the exon location shown is related to the specific RefSeq listed in the results table.
Assay ID:
".g" suffix indicates that the assay may detect genomic DNA when using either PrimeTime qPCR Assays (5’ nuclease probe-based assays) or PrimeTime qPCR Primers.
".gs" suffix indicates that the assay ordered as PrimeTime qPCR Assays (5’ nuclease probe-based assays) will not detect gDNA. Assay ordered as PrimeTime qPCR Primers may detect genomic DNA.
Exon Location:
Exon boundaries are consolidated at the gene level whenever possible. Some exon locations may be marked with a superscripted 1, indicating assays in which consolidating at the gene level is not possible. For these assays, the exon location shown is related to the specific RefSeq listed in the results table.
".g" suffix indicates that the assay may detect genomic DNA when using either PrimeTime qPCR Assays (5’ nuclease probe-based assays) or PrimeTime qPCR Primers.
".gs" suffix indicates that the assay ordered as PrimeTime qPCR Assays (5’ nuclease probe-based assays) will not detect gDNA. Assay ordered as PrimeTime qPCR Primers may detect genomic DNA.
Exon Location:
Exon boundaries are consolidated at the gene level whenever possible. Some exon locations may be marked with a superscripted 1, indicating assays in which consolidating at the gene level is not possible. For these assays, the exon location shown is related to the specific RefSeq listed in the results table.
Compare Assays
Gene Symbol
Species
Exon Location
Amplicon
Transcripts Hit
.g = Assay may detect genomic DNA when using either PrimeTime qPCR Assays (5’ nuclease probe-based assays) or PrimeTime qPCR Primers.
.gs = Assay ordered as PrimeTime qPCR Assays (5’ nuclease probe-based assays) will not detect gDNA. Assay ordered as PrimeTime qPCR Primers may detect genomic DNA.
Scale
Dye-Quencher Mod
N/A
Primer to Probe Ratio
N/A