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Introducing Rapid Genes NEW

Fast genes are in our DNA

Ready-to-use, NGS-verified clonal genes, perfect for quickly moving into your high throughput screening experiments.

Quality you can trust, industry leading speed you’ve come to count on.

Ordering

  • Rapid Genes ship in as soon as 5 business days
  • NGS-verified, clonal perfect gene synthesis
  • Available in cloning and expression vectors
  • Plate formats for high throughput workflows

Rapid Genes Gene Synthesis

Table 1. Available formats of Rapid Genes.

Plate typeBuffer optionVolumeConcentration
96-well PCR PlateNuclease-free water or IDTE pH 8.050 µL20 ng/µL
Dry (no buffer)N/AN/A
384-well PCR PlateNuclease-free water or IDTE pH 8.050 µL20 ng/µL
384-well Echo™ Plate**Nuclease-free water or IDTE pH 8.050 µL20 ng/µL

To achieve the fastest possible shipping time, items that do not meet IDT's purity and yield specifications after the first assembly attempt will be cancelled at no charge. No formal cancellation notice will be sent for these items. However, cancellations will be noted on the item specification sheet, which can be accessed using the Synthetic Biology Order Status Checker.

*Echo is a trademark of Labcyte Inc. A Beckman Coulter company

Product details

IDT Rapid Genes are NGS-verified, circular double-stranded DNA in a plasmid. They range in size from 125bp – 2000bp and ship as soon as 5 business days. They are available in a variety of cloning and expression vectors and ideal for high‑throughput screening workflows.

For Genes longer than 2kb or custom vectors order here.

Table 2. Vectors for Rapid Genes.

Vector nameSelection markerApplicationSequence
pUCIDT (Amp)AmpicillinCloningpUCIDT (Amp).txt
pUCIDT (Amp) Golden GateAmpicillinCloningpUCIDT (Amp) Golden Gate.txt
pUCIDT (Kan)KanamycinCloningpUCIDT (Kan).txt
pUCIDT (Kan) Golden GateKanamycinCloningpUCIDT (Kan) Golden Gate.txt
pIDTSmart (Amp)AmpicillinCloningpIDTSmart (Amp).txt
pIDTSmart (Kan)KanamycinCloningpIDTSmart (Kan).txt
pEXP-IDTAmpicillinMammalian ExpressionpEXP-IDT.txt
pET-IDTKanamycinBacterial ExpressionpET-IDT.txt
pET-IDT C HisKanamycinBacterial ExpressionpET-IDT C His.txt
pET-IDT Dual His + ThrombinKanamycinBacterial ExpressionpET-IDT Dual His + Thrombin.txt
pET-IDT N His + ThrombinKanamycinBacterial ExpressionpET-IDT N His + Thrombin.txt

They are available to ship dry or in resuspension buffer in 96-well or 384-well plates.

Table 3. Rapid Genes characteristics.

SpecificationInformation
Length 125bp-2000bp
QC 100% NGS verification
Shipping Time* 5-8 business days
Yield 1µg
Format Dry or resuspended in buffer at 20 ng/µl
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Request a consultation

Have questions for our experts? Want to onboard your own vector? Your time is valuable and we’ll prioritize your inquiry. Click on “Request a consultation” to provide brief information about your project, and we’ll be in touch to discuss it ASAP.

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Product data

Sequence verification

All inserts for Rapid Genes are sequence-verified by NGS. Upon delivery of the plasmids, an NGS-sequencing report, plasmid map, and FASTA file are all available to you in your online IDT account.

Frequently asked questions

Biosecurity

Sequence Information is secure and confidential at IDT. Please see our Confidentiality Statement for more information. All online ordering steps, including sequence entry and your choice of parameters, are also secure and protected.

We screen the sequence of every gene, gene fragment, and Megamer™ ssDNA fragment order we receive to (1) identify any regulated and other potentially dangerous pathogen sequences, and (2) verify that IDT’s gene customers are legitimate scientists engaged in beneficial research.

IDT is among the five founding members of the International Gene Synthesis Consortium (IGSC) and helped to create the IGSC’s Harmonized Screening Protocol. The Harmonized Screening Protocol describes the gene sequence and customer screening practices that IGSC member companies employ to prevent the misuse of synthetic genes. IDT takes the steps set out in the Harmonized Screening Protocol to screen the sequences of ordered genes and the prospective customers who submit those orders.

For more information about the IGSC and the Harmonized Screening Protocol, please visit the website at www.genesynthesisconsortium.org.

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In October 2010, the United States government issued final Screening Framework Guidance for Providers of Synthetic Double-Stranded DNA, describing how commercial providers of synthetic genes should perform gene sequence and customer screening. IDT and the other IGSC member companies supported the adoption of the Screening Framework Guidance, and IDT follows that Guidance in its application of the Harmonized Screening Protocol. For more information, please see 75 FR 62820 (Oct. 13, 2010), or https://federalregister.gov/a/2010-25728.

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