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Introducing Rapid Genes NEW
Fast genes are in our DNA
Ready-to-use, NGS-verified clonal genes, perfect for quickly moving into your high throughput screening experiments.
Quality you can trust, industry leading speed you’ve come to count on.
Ordering
- Rapid Genes ship in as soon as 5 business days
- NGS-verified, clonal perfect gene synthesis
- Available in cloning and expression vectors
- Plate formats for high throughput workflows
Rapid Genes Gene Synthesis
Table 1. Available formats of Rapid Genes.
| Plate type | Buffer option | Volume | Concentration |
|---|---|---|---|
| 96-well PCR Plate | Nuclease-free water or IDTE pH 8.0 | 50 µL | 20 ng/µL |
| Dry (no buffer) | N/A | N/A | |
| 384-well PCR Plate | Nuclease-free water or IDTE pH 8.0 | 50 µL | 20 ng/µL |
| 384-well Echo™ Plate* | Nuclease-free water or IDTE pH 8.0 | 50 µL | 20 ng/µL |
To achieve the fastest possible shipping time, items that do not meet IDT's purity and yield specifications after the first assembly attempt will be cancelled at no charge. No formal cancellation notice will be sent for these items. However, cancellations will be noted on the item specification sheet, which can be accessed using the Synthetic Biology Order Status Checker. Plates with less than 24 genes will incur an additional fee applied at checkout.
*Echo is a trademark of Labcyte Inc. A Beckman Coulter company
Product details
IDT Rapid Genes are NGS-verified, circular double-stranded DNA in a plasmid. They range in size from 125bp – 2000bp and ship as soon as 5 business days. They are available in a variety of cloning and expression vectors and ideal for high‑throughput screening workflows.
For Genes longer than 2kb or custom vectors order here.
Table 2. Vectors for Rapid Genes.
| Vector name | Selection marker | Application | Sequence |
|---|---|---|---|
| pUCIDT (Amp) | Ampicillin | Cloning | pUCIDT (Amp).txt |
| pUCIDT (Amp) Golden Gate | Ampicillin | Cloning | pUCIDT (Amp) Golden Gate.txt |
| pUCIDT (Kan) | Kanamycin | Cloning | pUCIDT (Kan).txt |
| pUCIDT (Kan) Golden Gate | Kanamycin | Cloning | pUCIDT (Kan) Golden Gate.txt |
| pIDTSmart (Amp) | Ampicillin | Cloning | pIDTSmart (Amp).txt |
| pIDTSmart (Kan) | Kanamycin | Cloning | pIDTSmart (Kan).txt |
| pEXP-IDT | Ampicillin | Mammalian Expression | pEXP-IDT.txt |
| pET-IDT | Kanamycin | Bacterial Expression | pET-IDT.txt |
| pET-IDT C His | Kanamycin | Bacterial Expression | pET-IDT C His.txt |
| pET-IDT Dual His + Thrombin.txt | Kanamycin | Bacterial Expression | pET-IDT Dual His + Thrombin.txt |
| pET-IDT N His + Thrombin | Kanamycin | Bacterial Expression | pET-IDT N His + Thrombin.txt |
They are available to ship dry or in resuspension buffer in 96-well or 384-well plates.
Table 3. Rapid Genes characteristics.
| Specification | Information |
|---|---|
| Length | 125bp-2000bp |
| QC | 100% NGS verification |
| Shipping Time* | 5-8 business days |
| Yield | 1µg |
| Format | Dry or resuspended in buffer at 20 ng/µl |
* Shipping time refers to the number of business days after order confirmation (excluding Fridays for wet plates). Shipping time is dependent on length and complexity of the Rapid Genes ordered. In a few cases, shipping time may exceed the estimated time.
Request a consultation
Have questions for our experts? Want to onboard your own vector? Your time is valuable and we’ll prioritize your inquiry. Click on “Request a consultation” to provide brief information about your project, and we’ll be in touch to discuss it ASAP.
Request a consultationProduct data
Sequence verification
All inserts for Rapid Genes are sequence-verified by NGS. Upon delivery of the plasmids, an NGS-sequencing report, plasmid map, and FASTA file are all available to you in your online IDT account.
Sample NGS report
Resources
Flyers
Certificates of analysis (COAs)
Frequently asked questions
Can rapid genes be ordered in my own vector?
No, but you can order standard genes in your own vector here.
Can Rapid Genes be ordered in tubes? Is there a minimum number required?
No, Rapid Genes are designed for high throughput workflows and are only delivered in plate formats. There is no minimum required but plates with less than 24 genes will incur an additional fee.
What storage conditions are recommended for Rapid Genes?
For short term storage <2 weeks 4ºC is recommended. For longer storage up to 2 years –20ºC
If my Rapid Gene fails to meet purity and yield specifications, will it be remade?
No, Rapid Genes are designed to achieve the fastest shipping time possible and items that do not meet these specifications after the first assembly attempt will be cancelled at no charge. These cancellations will be noted on the item specification sheet, which can be accessed using the Synthetic Biology Order Status Checker.
What plate formats are Rapid Genes available in?
Rapid Genes can be ordered in 96-well and 384-well PCR and ECHO plates.
Are Rapid Genes NGS verified?
Yes, they are NGS verified and clonally perfect.
When should I expect my Rapid Gene(s) to ship?
Depending on length and sequence complexity, they can ship in as soon as 5 business days. Longer, more complex sequences will require additional manufacturing times.
Biosecurity
Sequence Information is secure and confidential at IDT. Please see our Confidentiality Statement for more information. All online ordering steps, including sequence entry and your choice of parameters, are also secure and protected.
We screen the sequence of every gene, gene fragment, and Megamer™ ssDNA fragment order we receive to (1) identify any regulated and other potentially dangerous pathogen sequences, and (2) verify that IDT’s gene customers are legitimate scientists engaged in beneficial research.
IDT is among the five founding members of the International Gene Synthesis Consortium (IGSC) and helped to create the IGSC’s Harmonized Screening Protocol. The Harmonized Screening Protocol describes the gene sequence and customer screening practices that IGSC member companies employ to prevent the misuse of synthetic genes. IDT takes the steps set out in the Harmonized Screening Protocol to screen the sequences of ordered genes and the prospective customers who submit those orders.
For more information about the IGSC and the Harmonized Screening Protocol, please visit the website at www.genesynthesisconsortium.org.
In October 2010, the United States government issued final Screening Framework Guidance for Providers of Synthetic Double-Stranded DNA, describing how commercial providers of synthetic genes should perform gene sequence and customer screening. IDT and the other IGSC member companies supported the adoption of the Screening Framework Guidance, and IDT follows that Guidance in its application of the Harmonized Screening Protocol. For more information, please see 75 FR 62820 (Oct. 13, 2010), or https://federalregister.gov/a/2010-25728.
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