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OligoAnalyzer Tool

Know your oligos! The OligoAnalyzer Tool gives you critical insight into the behavior and properties of your desired sequences, before you order.

  • ✔ Length
  • ✔ GC content
  • ✔ Melting temperature (Tm)
  • ✔ Molecular weight
  • ✔ Secondary structure
  • ✔ Self-dimer & hetero-dimer tendencies
  • ✔ NCBI Blast

1. Enter your sequence

  • DNA
  • RNA
  • Locked nucleic acid bases
  • Modifications
  • Mixed bases
  • Phosphorothioated bases

2. Adjust calculation options

  • Hybridization target type
  • Oligo concentration
  • Na+ concentration
  • Mg2+ concentration
  • dNTP concentration
  • Use SpecSheet or qPCR preset values

3. Choose a function

  • Standard analysis
  • Hairpin
  • Self-dimer
  • Hetero-dimer
  • NCBI blast
  • Tm mismatch

OligoAnalyzer user manual

In-depth information on the tool’s functionality and features.

Help with terminology

A glossary of terms you will see when using the tool.

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