- Curated products—selected specifically for whole genome sequencing
- Short workflow—create a normalized library pool in under 4 hours
- Low input, high complexity—up to 3-fold fewer duplicates from 1 ng of DNA when using the xGen DNA Library Prep Kit EZ
- Enzymatic fragmentation—no need for specialized equipment or consumables for DNA fragmentation
- xGen Normalase™ technology—a proprietary enzymatic method of library normalization for multiplexed sequencing
- Automation friendly—works on a variety of platforms
What is whole genome sequencing?
Whole genome sequencing (WGS) provides comprehensive coverage over the entire mappable genome to gain information about an organism, or metagenomic DNA samples. In comparison to targeted sequencing which is often limited to a subset of genes of interest, WGS includes coverage of all coding and non-coding regions, regulatory sequences, and inter- and intra-genic portions of the genome. Then, the sequencing data are aligned to a reference genome for variant analysis, assembled into contigs for de novo genome assemblies, or used for microbial classification (in the case of metagenomic samples).
For low-throughput labs, we have a 16-reaction product solution for whole genome sequencing available that includes library prep and adapters. With this solution you will get a streamlined workflow consisting of three steps:
- Enzymatic preparation—DNA fragmentation, end-repair, and dA-tailing of dsDNA
- Adapter ligation
- PCR amplification
For library prep, the xGen DNA Library Prep Kit EZ uses an enzymatic fragmentation strategy to shear high-quality DNA samples. The kit employs TA ligation-based library prep, and includes an enzyme mix for fragmentation, end-repair and dA-tailing, a ligase to attach the xGen Stubby Adapter, and finally, a high-fidelity polymerase for indexing PCR. This solution offers premixed xGen UDI Primer Pairs (not included with the xGen DNA Library Prep Kit EZ) for indexing by PCR in order to generate an NGS library ready for Illumina® sequencing.
For high-throughput labs, we have a 96-reaction solution that can be used along with the xGen Normalase Module (sold separately), and includes a proprietary enzymatic library normalization kit for multiplexed sequencing. Using this method eliminates the need for individual library quantification while utilizing equal volume pooling rather than requiring different volumes for each library when making an equimolar library pool for multiplexed sequencing. Our Normalase enzymatic normalization method also results in more uniform read depth within a library pool, providing a coefficient of variation (CV) <10% when compared to methods that require individual library quantification