Processing
Genes & gene fragments
Luciferase, an alternative to fluorescence
Luciferase enzymes regulate bioluminescence in cells and can be found in a variety of organisms. It provides a useful research tool that allows for the study of gene regulation, among other things, and offers an alternative method to fluorescent techniques. Check out this DECODED article to find out what it is and how it can be used in the lab.
- Luciferase, an alternative to fluorescence
- The lac operon, explained
- Troubleshooting your site-directed mutagenesis by PCR
- Understanding the importance of 3’ UTR mutations in cancer research
- Gateway™ cloning: Definitions, tips, and tricks
- How DNA ligation works
- Primer design and other tools you should know about
- Using gBlocks™ HiFi Gene Fragments to explore novel pathways of antibiotic resistance
- Targeting antibiotic resistant microbial infections with synthetic biology
- Antigen-specific B cell enrichment for faster, more efficient antibody discovery
- The molecular characterization of new protein structures using eBlocks™ Gene Fragments
- Site-directed mutagenesis—Improvements to established methods
- Increase genetic stability with synthetic biology
- Characterization of antitumor CD8+ T cells in melanoma to optimize immune response
- Codon optimization tool makes synthetic gene design easy
- gBlocks™ Gene Fragments used in an antibody discovery workflow to test the efficiency of tumor-reactive antibodies
- Systems and synthetic biology: constructing smart and programmable microbes to address global problems
- CRISPR GUARD: A short gRNA-based system that facilitates precise genome edits
- Tips for designing spike-in controls for next generation sequencing analysis
- Tips for working with IDT Gene Fragments
- Cloning strategies: Blunt-end cloning
- Cloning strategies: Cohesive end cloning
- Site-directed mutagenesis methods
- Seamless assembly and cloning
