Custom rhAmpSeq Panels are designed for hotspot target SNPs and indels. We do not currently offer gene scanning or tiling panels, but we expect to offer panel design services for those applications in future product releases.
Custom rhAmpSeq Design Service
The 2 key steps in our custom design bioinformatics pipeline provide performance and workflow advantages over other amplicon sequencing systems.
- Assay design involves extensive target site review and
rhAmp primer design for each target, followed by comprehensive QC of each
rhAmp primer to mitigate off-target effects, such as primer-dimer formation and non-specific genomic hybridization.
- Virtual assay pooling is a unique multiplex primer QC feature that further enhances the specificity of
rhAmp PCR technology. It allows us to more accurately predict potential primer-dimer amplification products that could lower overall reaction efficiency and decrease the percentage of correctly mapped reads.
Custom rhAmpSeq Panels are available in 3 convenient scales to fit your experimental needs: 0.4 nmol, 4 nmol, and 8 nmol. As shown below (Table 1), we recommend adjusting
rhAmp primer concentrations in the first amplification reaction (Targeted rhAmp PCR 1) depending on your panel size (
plexity). Table 1 shows the approximate number of reactions for each scale based on panel size and scale.
Table 1. Number of rhAmp primer reactions based on scale and panel size.
* Important: When creating rhAmpSeq primer pools, do not to combine forward and reverse primers for long term storage. The primer concentrations in the 10X panel stocks assume making a separate forward pool of primers and a separate reverse pool of primers. Refer to the protocols in the Resources section for details.