PCR with RNase H (rhAmp PCR)
Reduce primer dimers and other undesirable amplification products with state-of-the-art PCR technology
What is rhAmp PCR?
rhAmp PCR is RNase H-dependent PCR (rhPCR), a nucleic acid amplification method that provides increased target specificity over traditional PCR. Compared to traditional PCR, rhAmp PCR requires an additional enzyme, RNase H2, and uses blocked primers (rhPrimers or rhAmp Primers) in place of conventional PCR primers.
How does it work?
rhAmp technology relies heavily on the unique design of rhPrimers and the inherent characteristics of RNase H2. As opposed to DNA-only primers used in traditional PCR and qPCR, rhPrimers contain a single RNA base and a 3′ blocking group that must be removed by RNase H2 before extension by DNA polymerase can occur (Figure 1). The added specificity achieved by this mechanism consistently yields more amplification of the fragments you are targeting, and less off-target amplification (Figure 2).
Who should use it?
The primer-dimer reducing abilities of rhAmp technology make it ideal for demanding genomics applications like multiplexed amplicon sequencing and SNP genotyping. In addition, if you are performing studies involving rare variant detection, splice variant discovery, or microbial identification, consider using rhAmp PCR to create your own PCR assay. Its added specificity can offer both efficiency and accuracy improvements to regular PCR when undesirable amplification artifacts are a consistent problem.