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  3. Gene expression
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Gene expression

We offer a selection of qPCR products to assess gene expression. Order a ready-to-use solution with PrimeTime™ Assays and master mix, or create a completely custom option from our probe catalog.

PrimeTime™ qPCR Probe Assays

PrimeTime qPCR Probe Assays are 5′ nuclease assays that include both predesigned or custom assays. Predesigned assays can be chosen from a catalog of options for human, mouse, and rat sequences. Custom assays may be created for all species using the PrimerQuest™ Tool. Predesigned and custom assays can be provided in tubes or plates.

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PrimeTime™ One-Step 4X Broad-Range Master Mix

PrimeTime One-Step 4X Broad-Range Master Mix is a ready-to-use 4X concentrated mixture that is designed for use in probe-based, real-time quantitative PCR. It is a premium one-step qPCR master mix that offers advanced capabilities including the option to amplify from crude or purified samples. It contains a hot-start reverse transcriptase, hot-start DNA polymerase, dNTPs, buffer components, and an enhancer solution which improves direct amplification from difficult samples.

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PrimeTime™ qPCR Primer Assays

PrimeTime qPCR Primer Assays for use with intercalating dyes, such as SYBR® Green dyes*, include predesigned primer sets for human, mouse, or rat sequences. Custom assays may be created for all other species using the PrimerQuest™ Tool. Predesigned and custom assays can be provided in tubes or plates.

* SYBR is a trademark of Thermo Fisher Scientific

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PrimeTime™ One-Step RT-qPCR Master Mix

The PrimeTime One-Step RT-qPCR Master Mix is a ready-to-use, 2X concentrated mixture that is designed for use in probe-based, real-time quantitative PCR. It contains an antibody-mediated hot-start DNA polymerase, reverse transcriptase, dNTPs, MgCl2, enhancers, and stabilizers. PrimeTime One-Step RT-qPCR Master Mix offers high quality results for routine one-step qPCR experiments.

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PrimeTime™ qPCR Probes

PrimeTime qPCR probes are customized to your specifications and include several probe types, including PrimeTime double-quenched probes, Affinity Plus LNA Probes, and molecular beacon probes. Additionally, MGB Eclipse® Probes (EliTech Group) and other probe types are available as GMP-compliant reagents.

*MGB Eclipse is a registered trademark of ELITech Group
** GMP refers to product manufactured in accordance with IDT’s ISO 13485: 2016 QMS

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PrimeTime™ Gene Expression Master Mix

PrimeTime Gene Expression Master Mix is a hot-start master mix delivering high quality results under either conventional or fast cycling conditions. Optimized for probe-based qPCR.

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Overview

Differences and changes in gene expression are important measures to understand biological systems (read the applications article, Gene expression analysis for further information). One of the most widely used methods of analyzing gene expression is quantitative PCR (qPCR) following cDNA synthesis. qPCR, also called real-time PCR, uses a fluorescent reporter molecule with a primer pair to measure target nucleic acid amplification during PCR cycling.

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