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Cas Nucleases & Nickases

Make CRISPR edits with confidence using our array of optimized Cas nucleases and nickases. With so many options to choose from, including wild-type, high-fidelity, and CGMP manufactured, we have you covered from the bench to the clinic.

Can’t decide which Cas enzyme you need?

Which Cas9 enzyme is right for you?

Alt-R S.p. Cas9 Nuclease Alt-R S.p. HiFi Cas9 Nuclease Alt-R S.p. Cas9 D10A Nickase Alt-R S.p. Cas9 H840A Nickase
Description Wild-type Cas9 with high genome editing potency that is simple to use and economical Cas9 variant with improved specificity based on reduced off-target effects, while preserving high on-target activity Cas9 variant with a mutation in the RuvC domain that disables cleavage of the non-target strand Cas9 variant with a mutation in the HNH domain that disables cleavage of the target strand
DNA cleavage Both strands Both strands Target strand Non-target strand
Suggested use First choice for most CRISPR genome editing projects Ideal for experiments sensitive to off-target events May be beneficial for homology-directed repair (HDR) experiments, but requires two suitable cutting sites within an optimal distance
Molecular weight 162,200 g/mol
Amount provided 100 μg or 500 μg
Concentration 10 mg/mL (62 μM) in 50% glycerol
Shipping conditions Dry ice
Storage conditions -20°C at stock concentration
Dilution Dilute in a medium (such as OptiMEM®) or Phosphate-buffered saline (PBS) before use

Minimize off-target edits with our HiFi Cas9 nuclease

Alt-R S.p. HiFi Cas9 Nuclease V3 facilitates near-WT editing potency while providing superior cutting specificity to reduce off-target editing.
chart showing reduced off-target site editing by hifi alt-r cas9
quality test data of gmp crispr reagents over 24 months

Consistent, reliable CGMP manufacturing for therapeutic development

Expect the best from Aldevron’s ISO13485-certified manufacturing process. (A) Quality tests show no loss of purity or (B) protein activity when the Cas nuclease is stored under recommended conditions.
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Unless otherwise expressly indicated in a Certificate of Analysis, label or other documentation accompanying the Products, the Products are intended for research use only and subject in each and every case to the condition that such sale does not convey any license, expressly or by implication, to manufacture, duplicate or otherwise copy or reproduce any of the Products.

SpyFi is the property of Aldevron and all information and data presented are solely those of Aldevron. Unless agreed to in writing, IDT does not warrant the fitness or suitability of these products. Purchaser is solely responsible for all decisions regarding the use of these products and any associated legal or regulatory obligations.

CGMP gRNA products are manufactured in accordance with ICH Q7, 21CFR210, 211, and parts of 600. IDT engineering runs and CGMP gRNA are for development and investigational use only. The performance characteristics of this product have not been established.  This product is not intended to be used as final drug product. The purchaser is solely responsible for all decisions regarding the intended use of the product and any associated legal or regulatory obligations.