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File Upload
Plate Upload Instructions
- Only one plate can be entered per tab
- Empty wells do not need to be indicated
- Download a sample Excel plate ordering template.
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1. Please specify an excel file.
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2. Review and select plates you would like to add
| Plate | # Wells | |
|---|---|---|
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click here for ordering instructions.
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Plate(s) contains errors, please review plates and fix any errors before proceeding.
Plate(s) contains warnings, please review wells for warnings and click the ignore warnings checkbox to proceed.
Ignore Warnings
Looking for controls?
NOTE:The same positive and negative controls can be used for both A.s. and L.b. Cas12a (Cpf1) nucleases.
To order, copy and paste the appropriate name and sequence from the following list into this ordering page:
Cas12a (Cpf1) positive control crRNAs
Item Name:
Cas12a (Cpf1) Pos Ctrl crRNA, Human HPRT
Sequence:
GGTTAAAGATGGTTAAATGAT
Item Name:
Cas12a (Cpf1) Pos Ctrl crRNA, Mouse HPRT
Sequence:
GGATGTTAAGAGTCCCTATCT
Item Name:
Cas12a (Cpf1) Pos Ctrl crRNA, Rat HPRT
Sequence:
ATGCTTAAGAGGTATTTGTTA
Cas12a (Cpf1) negative control crRNAs
Item Name:
Cas12a (Cpf1) Neg Ctrl crRNA #1
Sequence:
CGTTAATCGCGTATAATACGG
Item Name:
Cas12a (Cpf1) Neg Ctrl crRNA #2
Sequence:
CATATTGCGCGTATAGTCGCG
Item Name:
Cas12a (Cpf1) Neg Ctrl crRNA #3
Sequence:
GGCGCGTATAGTCGCGCGTAT
Help
Looking for controls?
NOTE:The same positive and negative controls can be used for both A.s. and L.b. Cas12a (Cpf1) nucleases.
To order, copy and paste the appropriate name and sequence from the following list into this ordering page:| Cas12a (Cpf1) positive control crRNAs | |
|---|---|
| Item Name: | Cas12a (Cpf1) Pos Ctrl crRNA, Human HPRT |
| Sequence: | GGTTAAAGATGGTTAAATGAT |
| Item Name: | Cas12a (Cpf1) Pos Ctrl crRNA, Mouse HPRT |
| Sequence: | GGATGTTAAGAGTCCCTATCT |
| Item Name: | Cas12a (Cpf1) Pos Ctrl crRNA, Rat HPRT |
| Sequence: | ATGCTTAAGAGGTATTTGTTA |
| Cas12a (Cpf1) negative control crRNAs | |
|---|---|
| Item Name: | Cas12a (Cpf1) Neg Ctrl crRNA #1 |
| Sequence: | CGTTAATCGCGTATAATACGG |
| Item Name: | Cas12a (Cpf1) Neg Ctrl crRNA #2 |
| Sequence: | CATATTGCGCGTATAGTCGCG |
| Item Name: | Cas12a (Cpf1) Neg Ctrl crRNA #3 |
| Sequence: | GGCGCGTATAGTCGCGCGTAT |
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Base Notations
- Standard Bases, (A, C, G, T) are not case sensitive.
- Mixed Bases, expressed in UPPERCASE.
- RNA, expressed as 'r_', for example rA, rU.
- Base notation for Affinity Plus (locked nucleic acids) = +A, +G, +C, +T
- 2' O-methyl RNA bases are entered as 'm_'.
- Phosphorothioated DNA bases are entered as '_*'
- Phosphorothioated RNA bases are entered as 'r_*'.
- Phosphorothioated 2'-O-methyl RNA bases are entered as 'm_*'.
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