Amplicon sequencing product family icon

xGen™ Custom Amplicon Panels

Targeted sequencing of your most important genes

Tailored to meet your research needs, custom amplicon panels for Illumina ™ and Oxford nanopore technologies ™ offer a completely curated targeted sequencing workflow to interrogate genomic targets relevant to you.

xGen NGS—made to discover.

Ordering

  • Multiplex hundreds to thousands of targets in a single tube PCR
  • Compatible with low-input samples using 2.5-hour workflow
  • xGen Normalase™ compatible: Supports high-throughput library quantification in a single enzymatic reaction
  • Designed for researching germline and somatic variants, or targeted pathogen sequencing
  • Provides high on-target and coverage uniformity
  • Support available to help design your panel for Illumina or ONT sequencers

Get NGS Amplicon Support

xGen Custom Amplicon Panels

This technology enables design of custom panels for targeted sequencing, offering a completely curated, targeted NGS workflow to rapidly interrogate genomic targets relevant to your research. xGen Amplicon technology’s ability to generate super amplicons result in increased coverage, even in changing or diverse genomes. Optional xGen™ Normalase module included to support high-throughput library quantification in a single enzymatic reaction.

Core Kit and Indexing sold separately.

Create designs for custom amplicon panels

To submit a design request, use the form and template. All custom panels are formatted in 384 rxn. Customers can choose a pool and ship option or a pool, and ship option with functional NGS testing and panel optimization.

Design request

Order my design

Upload your predesigned sequences by logging into your IDT customer account and filling out our order form. All custom panels are formatted in 384 rxn.

ORDER REQUEST

Any xGen Custom Amplicon Panel and Indexes for Oxford Nanopore Technologies™ will require a custom part number to be generated at point of order.

Figure 1. Plate combinations for 1536 UDI primer pairs. Each 96-well plate is sold in a set of four plates. For 1536 UDI primers, order Set 1, 2, 3, and 4.

For plate layouts of each of the 16 UDI plates offered see the xGen™ Normalase™ Indexing Primers quick reference guide.

xGen Custom Amplicon HS Panel

This technology enables low-frequency variant identification ≤1% through the incorporation of molecular identifiers (MIDs or UMIs), to reduce the rate of false-positive variant calls and increase variant identification introduced during PCR and sequencing.

Create designs for custom amplicon panels

To submit a design request, use the form and template. All custom panels are formatted in 384 rxn. Customers can choose a pool and ship option or a pool, and ship option with functional NGS testing and panel optimization.

Design request

Order my design

Upload your predesigned sequences by logging into your IDT customer account and filling out our order form. All custom panels are formatted in 384 rxn.

ORDER REQUEST

Any xGen Custom Amplicon Panel (HS included) will require a custom part number to be generated at point of order.

For customers who wish to purchase the legacy rhAmpSeq™, please contact us today.

Icons_Ocean_85x85_Support

Request a consultation

Want to learn more about our xGen™ Amplicon Panels—developed with super amplicon technology—and how to customize your panel or spike-in genes in our predesigned panels? Your time is valuable—we’ll prioritize your inquiry and be in touch to discuss it ASAP.

Request a consultation

Product details

xGen Custom Amplicon Panels are custom-designed primer pools to create multiple overlapping amplicons for specific genetic targets in research studies (Figure 1). Panels can multiplex hundreds to thousands of targets in a single tube using a simple 2.5-hour workflow (Figure 2). Compatible with cell-free DNA and FFPE (formalin-fixed paraffin embedded) research samples starting with as little as 10 ng input or pathogen RNA/DNA in the picogram range. For a listing of the specifications for custom panels, see Table 1. If multiple libraries are being examined at the same time, the final NGS library can be normalized using the IDT proprietary xGen Normalase reagent.

The cost-effective and flexible custom design means the panel can be used for research applications such as:

  • Genotyping by sequencing
  • Variant identification with a limit of detection down to 1%
  • Identifying germline inherited SNPs and indels
  • Somatic cell variant identification
  • Targeted virus or pathogen sequencing
  • Genetic fingerprinting
  • Liquid biopsy

xGen Custom Amplicon Panels

Icons_Ocean_85x85_Scalability
EFFICIENT NGS THROUGHPUT & SCALABILITY
Icons_Ocean_85x85_Speed
SIMPLEST, FASTEST WORKFLOW AVAILABLE
Icons_Ocean_85x85_Specificity-Precision
TAILORED GENOMIC CONTENT FOR LIBRARY PREP

xGen Custom Amplicon Panels are used to create targeted NGS libraries for research studies. Whether you need to examine hundreds or thousands of targets, the xGen Custom Amplicon Panels can be tailored to your specific research needs. Once created, the panel is used in a simple, one-tube workflow that is compatible with high-throughput applications.

Table 1. Product specifications of the xGen Custom Amplicon Panels

Feature Specification
Input DNA range 10–25 ng of amplifiable DNA
Amplicon size Versatile size for compatibility with many research sample types: whole blood, cell culture, FFPE, cfDNA (default maximum is 150 bp)
Custom design coverage >90% of requested bases
Workflow time Single-tube with 2.5-hour DNA-to-library workflow
Components provided Custom primer pool
Limit of detection As low as 1% allele frequency for variants
Panel size range 15–1500 amplicons per panel; other options available upon request
Library multiplexing capability Depends on type of indexing primers used
On-target %>90% on-target reads
Coverage uniformity >80% coverage uniformity at a >0.2 of mean depth
Compatible platforms Illumina® Oxford Nanopore Technologies™

 

Simple, 2.5-hour workflow

From DNA to NGS library in as little as 2.5 hours, the workflow for the xGen Custom Amplicon Panels can be done in a single tube with minimal hands-on steps. As shown in Figure 2, the DNA sample is first amplified with the multiplex custom panel to create copies of each of your intended targets. Then the amplicons are converted into indexed libraries in a second PCR reaction with the appropriate indexing primers for your NGS instrument. When multiple libraries are pooled, IDT-proprietary xGen Normalase reagent is used to normalize the libraries for NGS analysis.

Figure 2. xGen Custom Amplicon Panels have a single tube workflow that is done in as little as 2.5 hours. Creating an NGS library starts with multiplex PCR. Your custom panel is combined with the DNA sample to amplify the targets of interest. The samples are then amplified with indexing primers to create a functional dual indexed library. As an optional step, the xGen Normalase reagent can be used after pooling multiple libraries to ensure each is equally represented in the final sample for the flowcell.

Frequently asked questions

RUO21-0352_002