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Alt-R® Genome Editing Detection Kit

For detection of on-target or known off-target CRISPR events in cultured cells

The T7 endonuclease I (T7EI) mismatch cleavage assay detects on-target genome editing and estimates genome editing efficiency in CRISPR-treated cells.

  • Straightforward—use standard molecular biology techniques
  • Fast—does not require purification of PCR products before analysis
  • Consistent—analyze easy-to-read electrophoresis results
  • Quantifiable—gel band intensities relate to genome editing efficiency
  • Versatile—convenient for a single sample or high throughput analysis in plate format