icon_optional_services

Purification

For demanding applications such as multiplex PCR, cloning, mutagenesis or antisense/RNAi methods, additional purification can significantly improve oligonucleotide performance. Purified oligos up to 60 bases that are provided with a purity guarantee receive QC by capillary electrophoresis (CE). The traces are available online in your account order history.

Purification services offered include PAGE and various types of HPLC. Purified, unmodified oligos 20–50 bases in length are provided with the guaranteed yields (Table 1).*

Table 1. Yield guarantees for unmodified oligos of 20–50 bases, after purification.

Purification type100 nmol250 nmol1 µmol5 µmol10 µmol
PAGE1 OD2 OD10 OD50 OD100 OD
HPLC1 OD
4 OD20 OD100 OD200 OD
IE-HPLC1 OD
4 OD20 ODs100 OD200 OD
RNase-Free HPLC1 OD
4 OD20 ODs100 OD200 OD
Dual HPLC1 OD
2 OD10 ODs50 OD100 OD
Dual PAGE & HPLC0.5 OD1 OD5 ODs25 OD50 OD

* Purity and yield guarantees vary with oligo length, modifications, or sequence composition.