gene_expression

Molecular beacon probes

Improved signal-to-noise ratio with FRET-based hybridization probes

Molecular Beacons are dual-labeled probes that form a quenched, stem-loop structure in native state and fluoresce upon hybridization to the target nucleotide sequence. Applications include real-time and endpoint PCR, SNP detection, and multiplex amplification.

  • Detect single mismatch from target with hybridization probes
  • Perform post-PCR melt curve analysis, if needed, since probes are not hydrolyzed for signal generation
  • Choose from a wide variety of reporter and quencher combinations
  • Start experiments sooner: standard probes ship in 5–7 business days