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OligoAnalyzer™ Tool

Understand the expected behavior of your oligos before you order them.

  • Quickly see GC content, melting temperature, and more
  • Identify secondary structure potential
  • Minimize dimerization
  • Use NCBI Blast
OligoAnalyzer screenshot

Research-friendly UX

Flexible input and advanced parameters to optimize your custom order:

  • Enter your sequence
  • Adjust calculation options
  • Choose a function
Get started

New to the OligoAnalyzer tool?

Our video will introduce you to the basics and get you up and running quickly. We’ll go over the various functionalities available in the tool, using example sequences.

Creating optimized custom oligos just got easier

Icons Library_Sky_85x85_Build-Assemble
Enter your sequence

  • DNA
  • RNA
  • Locked nucleic acid bases
  • Modifications
  • Mixed bases
  • Phosphorothioated bases
Icons Library_Sky_85x85_Amplification
Adjust calculation options

  • Hybridization target type
  • Oligo concentration
  • Na+ concentration
  • Mg2+ concentration
  • dNTP concentration
  • Use SpecSheet or qPCR preset values
Icons Library_Sky_85x85_How to order
Choose a

  • Standard analysis
  • Hairpin
  • Self-dimer
  • Hetero-dimer
  • NCBI blast
  • Tm mismatch


Frequently asked questions

Web resources

OligoAnalyzer user manual

In-depth information on the tool’s functionality and features.

Help with terminology

A glossary of terms you will see when using the tool.

Need help or inspiration?

If you need further assistance please call, email, or web chat us today.

Contact us