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CRISPR genome editing

CRISPR knock-out: Tips and tricks

For CRISPR gene knock-outs, it’s important to design the right guide RNA to increase genome editing efficiency. For a successful knock-out, the Cas nuclease makes a double strand break (DSB) which is repaired through the non-homologous end joining pathway (NHEJ). Read more about the tips and tricks you need to know to increase the efficiency of your CRISPR experiments.