The xGen™ Hybridization and Wash Kit v2 is designed for use with xGen Hyb Panels and xGen Universal Blockers. This kit consists of two core components—the xGen Hybridization & Wash v2 Reagents and the xGen Hybridization & Wash v2 Beads—
to perform the hybridization capture workflow. The latest version of the kit contains a new, internally developed, xGen 2x Hi-Fi PCR Master Mix and is compatible with the protocol, xGen hybridization capture of DNA libraries for NGS target enrichment, to provide a complete, high-quality hybridization capture solution for targeted NGS research.
Prevent adapter cross-hybridization by using xGen Universal Blockers in your hybridization capture reactions. Adapter sequences are ligated to all library fragments, both on-target and off-target, before enrichment. These adapter sequences can hybridize
with each other during enrichment, creating a "daisy-chain" effect, with off-target fragments being captured alongside on-target fragments, which can impact sequencing efficiency.
xGen Universal Blockers bind to platform adapter sequences on a designated strand (usually the inverse of the synthetic adapter) to help prevent non-specific binding. Blocking the adapter sequences significantly improves capture results [1, 2].
We have partnered with several instrument vendors to develop automation scripts for research using the xGen hybridization capture protocol on liquid handling robots. Scripts are currently available for the following:
- Beckman Coulter Biomek™ i5 and i7 Automated Workstations
- Hamilton NGS STAR™ for Library Prep Workstation
- Perkin Elmer Sciclone™ Workstation
Please contact the instrument vendors for more information regarding script availability and installation or contact IDT Customer Care for an updated list of qualified automation scripts for liquid handling