gene_expression

Genotyping

Researchers often look for genetic variations to better understand phenotypic traits. Within a genome differences may arise in numerous ways, including single nucleotide polymorphisms (SNPs), insertions or deletions (indels), or copy number variations (CNV). Genotyping techniques use polymerase chain reaction (PCR), often with a primer-pair and target-specific fluorescent probe in quantitative PCR (qPCR), a sensitive and specific way to detect SNPs. Learn more about genotyping.

IDT offers a complete SNP genotyping solution, with predesigned assays, as well as complementary easy- and ready-to-use reagent mixes. For other applications, modified probes are available that can be incorporated into custom genotyping assays.


IDT offers a complete SNP genotyping solution, with predesigned assays, as well as complementary easy- and ready-to-use reagent mixes.

rhAmp SNP Genotyping System »

The precise, easy-to-use rhAmp SNP Genotyping System is a fully integrated genotyping solution that includes an extensive predesigned assay collection, a custom design tool, optimized reagent mixes, and optional synthetic control templates. SNP detection may be performed on any commonly available qPCR instrument. This system offers an out-of-the-box solution for SNP genotyping studies for small discovery or large screening projects.


For other applications, modified probes are available that can be incorporated into custom genotyping assays.

MGB SNP Genotyping Assays »

MGB SNP Genotyping Assays are composed of primers and shortened probe sequences with high specificity. The probes are dual-labeled, 5’ nuclease probes incorporating a 3′ conjugated MGB ligand, which stabilizes its complementary sequence duplex, thereby increasing Tm.

PACE™ SNP Genotyping Assays »

PACE™ SNP genotyping uses competitive, allele-specific PCR and a simple, easily detected fluorescent readout for bi-allelic genotyping. Obtain primer sets from IDT for cost-effective, high-throughput assays.

Affinity Plus qPCR Probes

Use Affinity Plus qPCR Probes for SNP genotyping, transcript variant identification, and more sensitive target detection in challenging samples (FFPE tissue, biofluids). The Affinity Plus bases used in these qPCR probes are locked nucleic acid monomers. When incorporated into a probe, they impart heightened structural stability, leading to increased hybridization melt temperature (Tm).

PrimeTime qPCR Probes »

These 5′ nuclease probes include a fluorescent reporter dye and 1 or 2 quencher dye(s). Select from a broad catalog of synthesis scales, delivery formats, and reporter dye–quencher combinations. Use MGB Probes to create shortened sequences with high specificity. The MGB probes are dual-labeled, 5’ nuclease probes incorporating a 5′ conjugated MGB ligand, which stabilizes its complementary sequence duplex, thereby increasing Tm.

Probes and primers manufactured to GMP standards »

Our GMP manufacturing services offer convenient, online ordering of small- to mid-scale synthesis of double-quenched probes and primers for gene expression and genotyping. Use MGB Eclipse® Probes* to create shortened probes with high specificity for use in GMP-regulated tests. Pair with primers manufactured under GMP regulations for a complete qPCR or genotyping assay.

* MGB Eclipse is a trademark of Elitech Group.