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Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

How long can I leave my qPCR mix (containing PrimeTime Gene Expression Master Mix) at room temperature and still generate consistent results?

The thermal stability of PrimeTime™ Gene Expression Master Mix makes it ideal for overnight PCR runs and high throughput experiments using robotic liquid-handling systems. We have obtained reliable results when qPCR was run immediately after setup, or after the reactions were maintained at room temperature up to 72 hr. For more data, see our white paper, Ambient shipping of PrimeTime Gene Expression Master Mix.