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xGen™ for Ultima Genomics®—Legacy

Trusted IDT xGen NGS chemistries for emerging sequencers

These products are legacy versions of IDT’s xGen for Ultima Genomics®, and are intended for use by existing customers only.

xGen NGS—made platform-agnostic

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xGen™ for Ultima Genomics® 

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Product data

Methyl-seq library preparation workflow comparisons

The xGen Methyl-Seq DNA Library Prep Kit has a post-bisulfite library preparation workflow to maximize library complexity by converting bisulfite-induced single-stranded fragments directly into library molecules. Traditional methods construct libraries from dsDNA using methylated adapters followed by bisulfite conversion that leads to significant library loss due to bisulfite-induced DNA fragmentation. The random priming methods are also post-bisulfite but have reduced library complexity due to a lower efficiency biased workflow (see Table 2; Figure 3).

References

  1. Jones PA. Functions of DNA methylation: islands, start sites, gene bodies and beyond. Nat Rev Genet. 2012 May 29;13(7):484-92. doi: 10.1038/nrg3230. PMID: 22641018.
  2. Luo C, Keown CL, Kurihara L, et al. Single-cell methylomes identify neuronal subtypes and regulatory elements in mammalian cortex. Science (New York, NY). 2017;357(6351):600-604.
  3. Luo C, Rivkin A, Zhou J, et al. Robust single-cell DNA methylome profiling with snmC-seq2. Nat Commun. 2018;9(1):3824.
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