Using next generation sequencing (NGS) to analyze SARS-CoV-2 can help researchers understand COVID-19. IDT, in partnership with the ARTIC Network, is offering their latest design of the ARTIC SARS-Co-V-2 Amplicon Panel to support global availability of this important research tool.
The ARTIC network—a group of researchers who are developing a system for processing different virus samples to generate public health information—has developed primer sequences, experimental recommendations, and bioinformatic resources to facilitate NGS analysis of the SARS-CoV-2 genome. Their primer design pipeline (PrimalSeq), previously deployed for other infectious disease sequencing, was especially useful early in the SARS-COV2 pandemic .
ARTIC provides a detailed experimental protocol featuring a set of primers for amplicon sequencing to quickly sequence SARS-CoV-2 . They also maintain a resource that alerts researchers to updates in the design . An adaptative protocol that allows for sequencing on Illumina sequencers have been developed by others .
The sequence of the SARS-CoV-2 genome can enable research in many areas, including:
- Origin identification
- Mutations and viral evolution rates
- Transmission routes of variants
- Population surveillance, such as wastewater-based epidemiology.