How to design qPCR primers Spanning exon junctions using the PrimerQuest tool
This tutorial demonstrates how to use PrimerQuest to design PCR or qPCR primers that span exon junctions for a gene. PrimerQuest is one of IDT's scientific design and analysis tools. It is the design tool of choice for custom design of primers and probes PCR, qPCR, and sequencing.
All Next generation sequencing videos