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Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

I transfected the cells with fluorescently labeled Alt-R™ CRISPR-Cas9 tracrRNA. When should I sort the cells by fluorescence activated cell sorting (FACS)?

FACS-sort cells within 24 hours [1] after delivery of the ribonucleoprotein (RNP) complex containing fluorescently labeled Alt-R™ CRISPR-Cas9 tracrRNA [ATTO™ 550, ATTO 488, or ATTO 647 (ATTO-TEC, GmbH)]. Data and details on use and function of the fluorescently labeled tracrRNA are included in our Application Note, Fluorescently labeled tracrRNA provides efficient genome editing while allowing cellular microscopy and FACS analysis.

1. Cossarizza A, Chang HD, Radbruch A, et al. Eur J Immunol. 2017;47(10):1584-1797.