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Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

How much fluorescently labeled tracrRNA should I use in my CRISPR-Cas9 experiments?

The amount of fluorescently labeled Alt-R™ CRISPR-Cas9 tracrRNA [ATTO™ 550, ATTO 488, or ATTO 647 (ATTO-TEC, GmbH)] needed varies by application and transfection method. Here are our recommendations. Use these as starting points as you optimize your experiments.

  FACS analysis Fluorescence microscopy
Lipofection ~10 nM 10 nM
Electroporation ≥0.5 µM
~0.5 µM