The amount of Alt-R® CRISPR-Cas9 tracrRNA – ATTO™ 550 needed varies by application and transfection method. Here are our recommendations. Use these as starting points as you optimize your experiments.
Table. Final concentration of Alt-R® CRISPR-Cas9 tracrRNA – ATTO™ 550 to use in transfection
|FACS analysis||Fluorescence microscopy|
|Lipofection||~10 nM||10 nM|