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The amount of Alt-R® CRISPR-Cas9 tracrRNA – ATTO™ 550 needed varies by application and transfection method. Here are our recommendations. Use these as starting points as you optimize your experiments.
Table. Final concentration of Alt-R® CRISPR-Cas9 tracrRNA – ATTO™ 550 to use in transfection
FACS analysis | Fluorescence microscopy | |
---|---|---|
Lipofection | ~10 nM | 10 nM |
Electroporation | ≥0.5 µM |
~0.5 µM |