- Highly versatile—These double-stranded DNA fragments of up to 3 kb can be used to easily and reliably assemble almost any sequence, and are compatible with most published cloning methods, including the Gibson Assembly® Method, and blunt-end or cohesive-end cloning protocols.
- Easy isothermal gene assembly—Using the Gibson Assembly Method, multiple gBlocks Gene Fragments can be assembled into a larger gene construct, in a single reaction that takes about 1 hour. A simple, 20–80 nt sequence overlap is required when designing the gene fragments for assembly.
- Affordable—gBlocks Gene Fragments are up to half the price of other synthetic gene constructs, making synthetic biology accessible to any lab.
- Short delivery time—gBlocks Gene Fragments are typically shipped within 5–8 business days.
High-fidelity, double-stranded DNA sequences
gBlocks Gene Fragments are sequence-verified, double-stranded DNA fragments, 125–3000 bp in length, that enable easy gene construction or modification. They are synthesized using the highest fidelity synthesis methods, developed by IDT for our industry-leading Ultramer® Oligonucleotides, making them ideal for a broad range of cloning techniques.
Since they were introduced in 2012, researchers have been using gBlocks Gene Fragments for many unique applications. The Church laboratory at Harvard University (Boston, MA, USA) published a protocol for designing gRNAs from gBlocks Gene Fragments, and transferring these gRNAs and Cas9 constructs into human-induced pluripotent stem cells and HEK293 cells . gBlocks Gene Fragments and the Gibson Assembly® method  can also be used as a reliable method for construct assembly when commercial clones to a poorly characterized gene are unavailable, such as in the case of the Micalcl protein, as described by Marbiah MM, Harvey A, et al .
Screen fewer clones
Experimental data shows that 90% of cloned gBlocks Gene Fragments will have the correct sequence (Figure 1).
In addition, using the Gibson Assembly method, two or more gBlocks fragments can easily be assembled in a single reaction to generate larger constructs  (Table 1). gBlocks Gene Fragments are synthesized without a 5′ phosphate; optional 5′ phosphorylation is available for blunt cloning.
|Wild-type clones||Gene length (nt)||gBlocks Gene Fragments (#)||Correct clones|
Get gBlocks Gene Fragments quickly
The easy online ordering system allows sequences for gBlocks Gene Fragments to be entered individually or as multiple entries using a provided Microsoft Excel template. Orders typically ship within 5–8 business days. gBlocks Gene Fragments are delivered with a short protocol that summarizes cloning methods for experienced users. Complete assembly and cloning protocols are available under Resources at www.idtdna.com/gblocks.