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Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

Why is TruGrade processing useful in my NGS experiments?

TruGrade® Processing reduces the risk of oligonucleotide crosstalk during multiplex next generation sequencing (NGS) applica­tions. Oligo crosstalk is a potential cause of barcode misalignment, which can lead to inaccurate conclusions drawn from associat­ing sequencing data with the wrong sample.

We recommend TruGrade Processing for sample preparation using barcoded adapt­ers for multiplex sequencing, and for PCR using barcoded fusion primers for multiplex amplicon sequencing.