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Alt-R HDR Donor Blocks offer a low-cost, high-fidelity option to create CRISPR-mediated insertions. We attain high-fidelity by creating a plasmid-derived clonal product which is confirmed to be correct by a standard NGS verification of this intermediate. This NGS analysis is performed on every HDR block. The final stage of production creates a linear product with modified ends using IDT’s proprietary method. Based on the hundreds of items produced and tested with this method we see no detectable difference between the sequence composition of the intermediate and the sequence composition of the final product. For most of our customers the additional cost and time associated with an additional round of sequencing is not useful.
For customers who wish to have an added level of quality control we offer an optional NGS service on the final product. This optional QC adds approximately 3 business days to the turnaround time and costs an additional $95 USD within the US, or the equivalent in your local currency.