Why are the standard NGS reports of the Alt-R™ HDR Donor Blocks of 2000 base pairs or shorter, titled differently than those longer than 2000 base pairs?
Alt-R HDR Donor Blocks offer a low-cost, high-fidelity option to create CRISPR-mediated insertions. We attain high fidelity by first creating a plasmid-derived clonal product which is confirmed to be correct by the standard NGS verification of this intermediate. This NGS is performed on every HDR block.
The next steps of processing are slightly different depending on the length of the sequence. The method used for sequences shorter than 2000 bp helps IDT to produce the items with a slightly faster turnaround time; while the method used for sequences longer than 2000 bp helps IDT to produce items that have similar success and on-time delivery rates as our shorter products.
Alt-R HDR Blocks <2 kb = Linear Intermediate Product NGS Report
Alt-R HDR Blocks >2 kb = Clonal Intermediate NGS Report