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No, our v3 chemistry is optimized to perform uniformly across library input, panel sizes, and hybridization times for 70°C. Hybridizations performed at lower temperatures, such as 65°C, can be significantly impactful for different applications. We strongly recommend hybridization at 70°C, especially for large panels, high library inputs, and short hybridizations. Experienced users and investigators can further optimize and tailor the conditions for their specific needs.