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What is new about version 3 of the xGen™ Hybridization and Wash Kit?
Version 3 can be used in the same manner as previous versions of the xGen Hybridization and Wash kits. IDT has optimized the buffers to reduce the overall number of washing steps and ease the workflow. Customers are not required to change their xGen capture panels or libraries than previously used.
We also introduce an optional short hybridization period of only 1 hour. Customers can still use the standard 4- or 16-hour incubations. We recommend users empirically test the shorter hybridization time for their workflows.
We have also lowered the recommended input for hybridization capture to 100 ng of library instead of 500 ng.