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If a user would like to use a short hybridization time (1 to 2 hours instead of 4 to 16 hours), optimization may be needed. Results may be variable and will depend on the capture panel (design and target size), desired analysis, and sample type.
The recommended library input range is 100 ng to 6 µg of total library. Target input will be enriched, however, we recommend not exceeding 2.5 µg total library input for 1-hour hybridization times.
We also do not recommend short hybridization times using very large or very small panels (< 2 Mb, or >30 Mb).