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Frequently asked questions
Our team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
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I switched to xGen™ Hybridization and Wash v3 Kit and noticed a shift in my GC read distribution. Is this normal?
xGen Hybridization Wash v3 will produce an improved AT/GC balance to better reflect the library content. The buffer compositions have changed to minimize bias from hybridization, capture, and wash. Therefore, most users who switch from v1 & v2 to v3 may observe an increase in AT content relative to the GC content.
The post-capture AT vs. GC content is largely dictated by the input library and the panel. Therefore, the resulting GC content would be a combination of the interactions between the library, the panel, the workflow, and the post-capture amplification mix.