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Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

How much xGen™ Stubby Adapter and Unique Dual Index Primer Pairs should I use for library prep?

This can vary by library prep method; consult the specific protocol you are using for an exact input range.

Most of IDT’s library prep kits utilize a 5 μL input for either adapter ligation (full-length or stubby), or indexing PCR.



*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.