The amount of xGen Stubby Adapter and Unique Dual Index Primer Pairs you use depends on the library prep workflow and the amount of starting sample used to perform library prep. We recommend checking your specific protocol for the optimal amount of adapter and indexing primers to use.
For the Lotus DNA Library Prep kit, use 4 µL of xGen Stubby Adapter (diluted according to the table below) for the ligation step. For the library amplification step, use 5 µL of xGen Unique Dual Index Primer Pairs.
|DNA input (ng)||Adapter dilution||Stock concentration (µM)|
If diluting the xGen Stubby Adapter is required, we recommend using Nuclease-Free Duplex Buffer. Nuclease-Free Duplex Buffer contains salts, which help keep the adapter duplexed.