IDT's contact information has changed! Visit our Contact Us page for updated information.
Processing
Frequently asked questions
Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
How far apart should I design my two guides when using the Alt-R® nickases?
When designing crRNA pairs to use with the Alt-R nickases, we recommend the following:
- Distance between cleavage sites:
- PAM-out orientation (right image)
(light blue = genomic DNA, dark blue = protospacer, and orange = PAM sequence):
Categories:
CRISPR genome editing*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.