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Frequently asked questions
Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
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How can I use FAM™ to visualize the uptake of my oligo into a cell, and can it be added to phosphorothioate-modified bases?
Attaching a fluorescent label to monitor cellular update of oligos is very common. It does not matter whether FAM is attached 5', 3', or internally.
Generally modifications are added to the 5' end as synthesis is easier, and therefore often cheaper. FAM can be added to phosphorothioate modified bases.