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Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

How to design my CRISPR-Cpf1 crRNA guide sequence - correct orientation & strand?

Enter the 20–24 base DNA sequence downstream (3’ end) of the target Cpf1 PAM site (TTTV*), and then you are ready to continue. We typically recommend using a 21-nucleotide guide sequence.

The ordering tool will automatically convert the DNA sequence to RNA during the ordering process. If you are pasting your Cpf1 target site from an online design tool, make sure to verify the correct strand orientation before pasting the target sequence.


* V = A, C, or G

*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.