Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
Do I need to include the Electroporation Enhancer in my electroporation experiments if I am also including an Alt-R HDR Donor Block?
We generally do not recommend use of the Alt-R™ Cas9 Electroporation Enhancer when using Alt-R HDR Donor Blocks. The DNA template is typically sufficient to act as a carrier for improved Cas9 delivery, in addition of Electroporation Enhancer may increase cytotoxicity. However, this may require optimization for individual cell lines since the use of Electroporation Enhancer could still benefit some difficult-to-transfect cells. (See Successful CRISPR genome editing in hard-to-transfect cells.)