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Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

Do I need to include the Electroporation Enhancer in my electroporation experiments if I am also including an Alt-R HDR Donor Block?

We generally do not recommend use of the Alt-R™ Cas9 Electroporation Enhancer when using Alt-R HDR Donor Blocks. The DNA template is typically sufficient to act as a carrier for improved Cas9 delivery, in addition of Electroporation Enhancer may increase cytotoxicity. However, this may require optimization for individual cell lines since the use of Electroporation Enhancer could still benefit some difficult-to-transfect cells. (See Successful CRISPR genome editing in hard-to-transfect cells.)

*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.