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Do I have to use Normalase™ to finish my SNAP libraries?
No. After the indexing PCR step and the final bead-based size selection/clean up, SNAP libraries may be quantified with conventional methods such as Qubit®, Agilent Bioanalyzer®, qPCR followed by manual pooling, or another comparable method. Alternatively, they can be normalized enzymatically with the included Normalase reagents.