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Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

Can IDT synthesize oligos using trimer phosphoramidites (trinucleotides)?

Yes, IDT can synthesize oligonucleotides for customers using trimer phosphoroamidites. You can order Trimer 19 using modification code /iTriMix19/, or Trimer 20 using modification code /iTriMix20/. To order a Custom Trimer Mix, email with your name, organization, and sequence designs.

Oligos containing Custom Trimer Mixes can be ordered with standard desalt (recommended for oligos containing mixes), provided that other modifications included in the sequence do not require HPLC.

For more information about trimer mixes, see the article, Generate codon balanced libraries for mutagenesis with trimer modifications,or contact

Custom DNA & RNA