Processing
Use of NCBI databases in qPCR assay design
In this video, Dr Elisabeth Wagner, Scientific Applications Specialist at IDT, covers:
1. Which NCBI tools are useful for designing qPCR assays
2. Using tools for qPCR design in the IDT SciTools® suite
3. Features and tools available on the NCBI website
4. Obtaining sequence information for your gene of interest
5. Performing a BLAST search for assay specificity
6. Searching for SNPs
7. Understanding how to proceed with a basic qPCR design
This presentation was given on April 15th, 2014 at the qPCR Workshop at the University of Iowa BioVentures Center.
All general molecular biology videos
