SARS-CoV-2. Don’t let up. We’ll help.

Increasing genome editing efficiency and specificity with optimized CRISPR-Cas9 guide RNAs

Use of CRISPR-Cas9 has revolutionized targeted genome editing. The process requires the Cas9 enzyme and guide RNA (gRNA), which directs Cas9 to the target DNA location. The gRNA can be generated using a variety of methods. In this webinar, Ashley Jacobi compares the on-target and off-target editing events observed with different gRNA formats in transformed and primary cell lines. She provides guidelines on when to use each gRNA in the context of your Cas9 source (plasmid, mRNA, ribonucleoprotein complex) and cell type.

Published on: October 26, 2018

Categories:

Gene knockout Gene mutation Genome editing Homology-directed repair CRISPR genome editing Alt-R CRISPR-Cas9 Alt-R CRISPR nucleases Alt-R HDR Donor Oligos Cancer Research Webinar CRISPR-Cas systems Alt-R enzymes Alt-R CRISPR-Cas9 Alt-R other enzymes Detection of CRISPR edits Targeted sequencing Amplicon sequencing

Tags:

crispr cas9 genome editing CRISPR-Cas9 amplicon sequencing CRISPR genome editing homology directed repair guide RNA gRNA cas9 gRNA design