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7 Citations found

Handy SM, Timme RE, et al. (2013) Development of a locked nucleic acid real-time polymerase chain reaction assay for the detection of Pinus armandii in mixed species pine nut samples associated with dysgeusia. J Agric Food Chem, 61 : 1060–1066.

The group designed a PrimeTime qPCR LNA Probe targeting a P. armandii SNP sequence.The probe was 24 bases long and contained 6 LNA bases in addition to the 5′ 6-FAM label and 3′ black hole quencher (3BHQ_1). A second PrimeTime qPCR LNA Probe was designed to serve as a blocker probe for species of trees related to the species of interest, Pinus armandii. The probe was also 24 bases long with 6 LNA bases in addition to a 5′ HEX label and a 3′ BHQ.  These reporter dyes (HEX and FAM) can be spectrally resolved from each other because they emit at different wavelengths.

Yamashita F, Azuma K, et al. (2013) Prognostic value of EGFR mutation and ERCC1 in patients with non-small cell lung cancer undergoing platinum-based chemotherapy. PLoS One, 8 : e71356.

PNA (peptic nucleic acid-locked nucleic acid) clamp Primers and LNA (locked nucleic acid) mutant probes from IDT were used in a PNA-LNA PCR clamp assay for EGFR mutation analysis.

Owczarzy R, You Y, Groth CL, Tataurov AV. (2011) Stability and mismatch discrimination of locked nucleic acid-DNA duplexes. Biochem, 50 : 9352–9367.

New nearest-neighbor parameters correctly forecast the positive and negative effects of LNAs on mismatch discrimination. Specificity is enhanced in a majority of sequences and is dependent on mismatch type and adjacent base pairs.

Johnson MP, Haupt LM, Griffiths LR . (2004) Locked nucleic acid (LNA) single nucleotide polymorphism (SNP) genotype analysis and validation using real-time PCR. Nucleic Acids Res, 32 : e55.
Ugozzoli LA, Latorra D, et al. (2004) Real-time genotyping with oligonucleotide probes containing locked nucleic acids. Analytical Biochemistry, 324 : 143–152.
McTigue PM, Peterson RJ, Kahn JD. (2004) Sequence-dependent thermodynamic parameters for locked nucleic acid (LNA)-DNA duplex formation. Biochemistry, 43 : 5388–5405.

Improved modified probe design through consideration of modified nucleic acid thermodynamics.

Letertre C, Perelle S, et al. (2003) Evaluation of the performance of LNA and MGB probes in 5′-nuclease assays. Mol Cell Probes, 17 : 307–311.