Use of a DNA aptamer to replace monoclonal antibodies in an impedance biosensor that uses microfluidics and a microelectrode for detection of the target molecule, in this case, the H5N1 subtype of avian influenza virus. Aptamer secondary structures and delta G were calculated using the free IDT online UNAFold and OligoAnalyzer programs. Biotinylated aptamers were synthesized by IDT with biotin conjugated at the 5′ end.
A review of the use of aptamers to target siRNAs to HIV-1 proteins.
Description of a new aptamer selection platform that uses fixed-region blocking elements to ensure library diversity and guard against amplification artifacts. Truncated (random-region sequence only) and full-length library versions of aptamers were synthesized and HPLC-purified by IDT.
Development of the first agent for imaging and quantifying the cancer associated protein, tenascin-C—a tenascin-C-specific single-stranded DNA aptamer. The aptamer was radiolabeled with 18F and 64Cu and used in PET-imaging studies to measure tumor uptake and metabolism. All aptamers, including FITC-aptamers, were synthesized by IDT.
xGen Lockdown Probes were used to rescue drop-out regions of SureSelect® probe panels (Agilent), giving more uniform target coverage. The probes were able to target 29 genes from whole blood genomic DNA, isolated from over 1000 patients.
The authors used gBlocks® Gene Fragments to generate a standard curve for their qPCR analysis.
Next generation sequencing was used to accurately detect constitutional mosaicism in an individual diagnosed with early onset, high-grade breast cancer. xGen Lockdown Probes were used to target multiple genes of interest, including <em>BRCA1</em>, in DNA isolated from the individual’s blood.
gBlocks Gene Fragments were used to generate the fatty acyl-CoA reductase gene from barn owl.
xGen Lockdown Probes were designed to target and capture segments of DNA at the 8q24 locus, a chromosomal region known for being involved in prostate cancer susceptibility.
The specificity, uptake, and target binding strength of 2 DNA aptamers were investigated in glioma cells and patient tissue. Aptamers were synthesized by IDT and conjugated at the 5′ end with either Cy® 3 or biotin for purification.
xGen Lockdown Probes were used to target regions of the genome containing commonly mutated genes. The probes allowed the authors to achieve sequencing with more specificity and depth, which greatly improved the efficiency and cost of data collection.
Scientists at the University of Oxford used xGen Lockdown Probes in a new, high-throughput method for detecting and sequencing HCV genotypes.