Ultramer Oligonucleotides were used to generate standard curves for absolute quantification. Each Ultramer standard was designed to contain the 5′-end of a transcript under study and qPCR amplified using transcript-specific primers.
A portion of the second exon of herpes simplex virus, immediate early protein ICP0, was synthesized as a MiniGene synthetic gene. The MiniGene synthetic gene construct was subcloned into a secondary vector system and used to generate RNA for use as a standard in RT-qPCR.
IDT MiniGene constructs were designed as IRF2BP2 (Interferon regulatory factor 2 binding protein 2) mutants: 355 GAC GAC GAC GAC 358 (aspartic acids), TCT 360 TAT (S360A), and TCT 360 GAT (S360D). The MiniGene cosnstructs were flanked by endogenous PstI and NcoI in IRF2BP2 for subcloning by restriction digest.
Ultramer oligonucleotides were used to construct a new vector that provides one-step incorporation of the PBAD promoter in front of any gene.
New nearest-neighbor parameters correctly forecast the positive and negative effects of LNAs on mismatch discrimination. Specificity is enhanced in a majority of sequences and is dependent on mismatch type and adjacent base pairs.
Ultramer Oligonucleotides were used as standards due to their purity and quantification precision.
An IDT MiniGene expressing wild-type survival motor neuron from SMN2, and a second SMN2 MiniGene with a silent mutation in the siRNA recognition sequence, were used to study the effects of siRNA knockdown, and rescue, of SMN1/2 on gene splicing. IDT DsiRNA Duplexes were also used for the siRNA knockdown experiments.
A review summarizing the use of cell-internalizing aptamers to deliver siRNAs to target cells. Highlights the optimization and improvement of aptamer-targeted siRNAs for clinical translation.